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Reducing image distortions due to temperature-related microscope stage drift.

by: J. Adler, S. N. Pagakis
Journal of microscopy, Vol. 210, No. Pt 2. (May 2003), pp. 131-137.

X Abstract

Distortions in confocal 3D image data sets were related to movements of the microscope stage that matched fluctuations in laboratory temperature. Movement was apparent in all three axes. Adding a draught-proof enclosure, covering the stage, objective lenses and supporting structures, minimized these short-term fluctuations. However, the stage still tracked slow changes in laboratory temperature and was sensitive to heat sources mounted on the microscope. Suggestions are made about microscope design.

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