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Visualization of membrane bound cations by a fluorescent technique

by: A. H. Caswell, J. D. Hutchison
Biochemical and Biophysical Research Communications, Vol. 42, No. 1. (8 January 1971), pp. 43-49.

X Abstract

Chlorotetracycline binds to divalent diamagnetic cations in aqueous solution with enhanced fluorescence. The fluorescence of the chelate is polarity dependent, being higher in nonaqueous solvents. If biological membranes or detergent micelles which bind cations are suspended in an aqueous phase, the fluorescence of the chlorotetracycline chelate shows enhanced fluorescence over the aqueous complex indicating migration of the divalent cation and chlorotetracycline into the nonaqueous membrane region. The fluorescence of chlorotetracycline bound to mitochondria varies as a function of the respiratory state of the mitochondria.

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