CiteULike Search Register Log in
CiteULike is a free online bibliography manager. Register and you can start organising your references online.

The Extent to Which ATP Demand Controls the Glycolytic Flux Depends Strongly on the Organism and Conditions for Growth

by: Brian J. Koebmann, Hans V. Westerhoff, Jacky L. Snoep, Christian Solem, Martin B. Pedersen, Dan Nilsson, Ole Michelsen, Peter R. Jensen
Molecular Biology Reports, Vol. 29, No. 1. (1 March 2002), pp. 41-45.

X Abstract

Using molecular genetics we have introduced uncoupled ATPase activity in two different bacterial species, Escherichia coli and Lactococcus lactis, and determined the elasticities of the growth rate and glycolytic flux towards the intracellular [ATP]/[ADP] ratio. During balanced growth in batch cultures of E. coli the ATP demand was found to have almost full control on the glycolytic flux (FCC=0.96) and the flux could be stimulated by 70%. In contrast to this, in L. lactis the control by ATP demand on the glycolytic flux was close to zero. However, when we used non-growing cells of L. lactis (which have a low glycolytic flux) the ATP demand had a high flux control and the flux could be stimulated more than two fold. We suggest that the extent to which ATP demand controls the glycolytic flux depends on how much excess capacity of glycolysis is present in the cells.

View the full article here:

DOI

This article has been bookmarked once, on 2008-10-08.

2008-10-08 User athleem
Privacy Statement | Terms & Conditions
CiteULike organises scholarly (or academic) papers or literature and provides bibliographic (which means it makes bibliographies) for universities and higher education establishments. It helps undergraduates and postgraduates. People studying for PhDs or in postdoctoral (postdoc) positions. The service is similar in scope to EndNote or RefWorks or any other reference manager like BibTeX, but it is a social bookmarking service for scientists and humanities researchers.