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The antisense transcriptomes of human cells.

by: Yiping He, Bert Vogelstein, Victor E. Velculescu, Nickolas Papadopoulos, Kenneth W. Kinzler
Science (New York, N.Y.), Vol. 322, No. 5909. (19 December 2008), pp. 1855-1857.

X Abstract

Transcription in mammalian cells can be assessed at a genome-wide level, but it has been difficult to reliably determine whether individual transcripts are derived from the plus or minus strands of chromosomes. This distinction can be critical for understanding the relationship between known transcripts (sense) and the complementary antisense transcripts that may regulate them. Here, we describe a technique that can be used to (i) identify the DNA strand of origin for any particular RNA transcript, and (ii) quantify the number of sense and antisense transcripts from expressed genes at a global level. We examined five different human cell types and in each case found evidence for antisense transcripts in 2900 to 6400 human genes. The distribution of antisense transcripts was distinct from that of sense transcripts, was nonrandom across the genome, and differed among cell types. Antisense transcripts thus appear to be a pervasive feature of human cells, which suggests that they are a fundamental component of gene regulation.

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This article has been bookmarked 23 times, initially on 2008-12-05.

2009-09-30 User rsaito
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2009-01-02 Group Koch_Lab , 1 note

Combined with the other articles and perspective from this issue of Science, there is a lot of very cool and intriguing evidence for widespread antisense transcription that is correlated with genes (i.e., it is non-random).

"Antisense transcripts thus appear to be a pervasive feature of human cells, which suggests that they are a fundamental component of gene regulation."

"However, the identity of the S, AS, and SAS genes varied among the cell lines, which suggests that the expression of antisense tags may be regulated in a cell- or tissue-specific manner"

2009-01-02 18:25:53
User skoch3 , 1 note

Combined with the other articles and perspective from this issue of Science, there is a lot of very cool and intriguing evidence for widespread antisense transcription that is correlated with genes (i.e., it is non-random).

"Antisense transcripts thus appear to be a pervasive feature of human cells, which suggests that they are a fundamental component of gene regulation."

"However, the identity of the S, AS, and SAS genes varied among the cell lines, which suggests that the expression of antisense tags may be regulated in a cell- or tissue-specific manner"

2009-01-02 18:25:53
2008-12-25 Group Roswell Cancer Crosstalk
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