Genome-Scale Reconstruction of the Saccharomyces cerevisiae Metabolic Network

10.1101/gr.234503 The metabolic network in the yeast was reconstructed using currently available genomic, biochemical, and physiological information. The metabolic reactions were compartmentalized between the cytosol and the mitochondria, and transport steps between the compartments and the environment were included. A total of 708 structural open reading frames (ORFs) were accounted for in the reconstructed network, corresponding to 1035 metabolic reactions. Further, 140 reactions were included on the basis of biochemical evidence resulting in a genome-scale reconstructed metabolic network containing 1175 metabolic reactions and 584 metabolites. The number of gene functions included in the reconstructed network corresponds to â¼16% of all characterized ORFs in . Using the reconstructed network, the metabolic capabilities of were calculated and compared with . The reconstructed metabolic network is the first comprehensive network for a eukaryotic organism, and it may be used as the basis for in silico analysis of phenotypic functions. [Supplemental material is available online at . The detailed genome-scale reconstructed model of can be found at or.]