X-chromosome-wide profiling of MSL-1 distribution and dosage compensation in Drosophila. Export

@article{citeulike:578264, abstract = {In Drosophila, dosage compensation is achieved by a twofold up-regulation of the male X-linked genes and requires the association of the male-specific lethal complex (MSL) on the X chromosome. How the MSL complex is targeted to X-linked genes and whether its recruitment at a local level is necessary and sufficient to ensure dosage compensation remain poorly understood. Here we report the MSL-1-binding profile along the male X chromosome in embryos and male salivary glands isolated from third instar larvae using chromatin immunoprecipitation (ChIP) coupled with DNA microarray (ChIP-chip). This analysis has revealed that majority of the MSL-1 targets are primarily expressed during early embryogenesis and many target genes possess DNA replication element factor (DREF)-binding sites in their promoters. In addition, we show that MSL-1 distribution remains stable across development and that binding of MSL-1 on X-chromosomal genes does not correlate with transcription in male salivary glands. These results show that transcription per se on the X chromosome cannot be the sole signal for MSL-1 recruitment. Furthermore, genome-wide analysis of the dosage-compensated status of X-linked genes in male and female shows that most of the X chromosome remains compensated without direct MSL-1 binding near the gene. Our results, therefore, provide a comprehensive overview of MSL-1 binding and dosage-compensated status of X-linked genes and suggest a more global effect of MSL complex on X-chromosome regulation.}, address = {European Molecular Biology Laboratory, 69117 Heidelberg, Germany;}, author = {Legube, Ga\"{e}lle and McWeeney, Shannon K K. and Lercher, Martin J J. and Akhtar, Asifa}, citeulike-article-id = {578264}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gad.377506}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16547175}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16547175}, day = {17}, doi = {10.1101/gad.377506}, issn = {0890-9369}, journal = {Genes Dev}, keywords = {compensation, dosage, fly, x-chromosome}, month = {March}, posted-at = {2006-04-06 11:30:19}, priority = {3}, title = {X-chromosome-wide profiling of MSL-1 distribution and dosage compensation in Drosophila.}, url = {http://dx.doi.org/10.1101/gad.377506}, year = {2006} }

TY - JOUR ID - citeulike:578264 L3 - citeulike-article-id:578264 N2 - In Drosophila, dosage compensation is achieved by a twofold up-regulation of the male X-linked genes and requires the association of the male-specific lethal complex (MSL) on the X chromosome. How the MSL complex is targeted to X-linked genes and whether its recruitment at a local level is necessary and sufficient to ensure dosage compensation remain poorly understood. Here we report the MSL-1-binding profile along the male X chromosome in embryos and male salivary glands isolated from third instar larvae using chromatin immunoprecipitation (ChIP) coupled with DNA microarray (ChIP-chip). This analysis has revealed that majority of the MSL-1 targets are primarily expressed during early embryogenesis and many target genes possess DNA replication element factor (DREF)-binding sites in their promoters. In addition, we show that MSL-1 distribution remains stable across development and that binding of MSL-1 on X-chromosomal genes does not correlate with transcription in male salivary glands. These results show that transcription per se on the X chromosome cannot be the sole signal for MSL-1 recruitment. Furthermore, genome-wide analysis of the dosage-compensated status of X-linked genes in male and female shows that most of the X chromosome remains compensated without direct MSL-1 binding near the gene. Our results, therefore, provide a comprehensive overview of MSL-1 binding and dosage-compensated status of X-linked genes and suggest a more global effect of MSL complex on X-chromosome regulation. JF - Genes Dev SN - 0890-9369 AD - European Molecular Biology Laboratory, 69117 Heidelberg, Germany; TI - X-chromosome-wide profiling of MSL-1 distribution and dosage compensation in Drosophila. KW - compensation KW - dosage KW - fly KW - x-chromosome AU - Legube, Gaëlle AU - McWeeney, Shannon K AU - Lercher, Martin J AU - Akhtar, Asifa PY - 2006/03/17/ UR - http://dx.doi.org/10.1101/gad.377506 ER -