High-resolution ChIP-chip analysis reveals that the Drosophila MSL complex selectively identifies active genes on the male X chromosome. Export

@article{citeulike:578275, abstract = {X-chromosome dosage compensation in Drosophila requires the male-specific lethal (MSL) complex, which up-regulates gene expression from the single male X chromosome. Here, we define X-chromosome-specific MSL binding at high resolution in two male cell lines and in late-stage embryos. We find that the MSL complex is highly enriched over most expressed genes, with binding biased toward the 3' end of transcription units. The binding patterns are largely similar in the distinct cell types, with approximately 600 genes clearly bound in all three cases. Genes identified as clearly bound in one cell type and not in another indicate that attraction of MSL complex correlates with expression state. Thus, sequence alone is not sufficient to explain MSL targeting. We propose that the MSL complex recognizes most X-linked genes, but only in the context of chromatin factors or modifications indicative of active transcription. Distinguishing expressed genes from the bulk of the genome is likely to be an important function common to many chromatin organizing and modifying activities.}, address = {Howard Hughes Medical Institute;}, author = {Alekseyenko, Artyom A A. and Larschan, Erica and Lai, Weil R R. and Park, Peter J J. and Kuroda, Mitzi I I.}, citeulike-article-id = {578275}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gad.1400206}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16547173}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16547173}, day = {17}, doi = {10.1101/gad.1400206}, issn = {0890-9369}, journal = {Genes Dev}, keywords = {compensation, dosage, fly, x-chromosome}, month = {March}, posted-at = {2006-04-06 11:38:59}, priority = {3}, title = {High-resolution ChIP-chip analysis reveals that the Drosophila MSL complex selectively identifies active genes on the male X chromosome.}, url = {http://dx.doi.org/10.1101/gad.1400206}, year = {2006} }

TY - JOUR ID - citeulike:578275 L3 - citeulike-article-id:578275 N2 - X-chromosome dosage compensation in Drosophila requires the male-specific lethal (MSL) complex, which up-regulates gene expression from the single male X chromosome. Here, we define X-chromosome-specific MSL binding at high resolution in two male cell lines and in late-stage embryos. We find that the MSL complex is highly enriched over most expressed genes, with binding biased toward the 3' end of transcription units. The binding patterns are largely similar in the distinct cell types, with approximately 600 genes clearly bound in all three cases. Genes identified as clearly bound in one cell type and not in another indicate that attraction of MSL complex correlates with expression state. Thus, sequence alone is not sufficient to explain MSL targeting. We propose that the MSL complex recognizes most X-linked genes, but only in the context of chromatin factors or modifications indicative of active transcription. Distinguishing expressed genes from the bulk of the genome is likely to be an important function common to many chromatin organizing and modifying activities. JF - Genes Dev SN - 0890-9369 AD - Howard Hughes Medical Institute; TI - High-resolution ChIP-chip analysis reveals that the Drosophila MSL complex selectively identifies active genes on the male X chromosome. KW - compensation KW - dosage KW - fly KW - x-chromosome AU - Alekseyenko, Artyom A AU - Larschan, Erica AU - Lai, Weil R AU - Park, Peter J AU - Kuroda, Mitzi I PY - 2006/03/17/ UR - http://dx.doi.org/10.1101/gad.1400206 ER -