The XIAP IRES activates 3Ⲡcistron expression by inducing production of monocistronic mRNA in the βgal/CAT bicistronic reporter system Export

@article{citeulike:5949614, abstract = {10.1261/rna.1557809 X-chromosome linked inhibitor of apoptosis (XIAP) mRNA has been proposed to bear a stress-activated internal ribosome entry site (IRES) that stimulates translation under conditions that inhibit cap-dependent initiation. However, several reports have indicated that the strong activity of the XIAP IRES in certain bicistronic reporter assay systems stems from production of unintended monocistronic transcripts through splicing or cryptic promoter activity. Here we extend these findings by providing evidence that the XIAP IRES similarly provokes the production of monocistronic mRNA encompassing the 3\^{a}€² cistron in the \^{I}²gal/CAT bicistronic reporter plasmid that was originally used to identify and characterize this putative IRES, through cryptic promoter activity.}, author = {Saffran, Holly A. and Smiley, James R.}, citeulike-article-id = {5949614}, citeulike-linkout-0 = {http://dx.doi.org/10.1261/rna.1557809}, citeulike-linkout-1 = {http://rnajournal.cshlp.org/content/15/11/1980.abstract}, citeulike-linkout-2 = {http://rnajournal.cshlp.org/content/15/11/1980.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19713328}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19713328}, doi = {10.1261/rna.1557809}, journal = {RNA}, keywords = {ires, mrna, promoter\_activity, x\_chromosome}, month = {November}, number = {11}, pages = {1980--1985}, posted-at = {2009-10-16 09:02:07}, priority = {2}, title = {The XIAP IRES activates 3\^{a}€² cistron expression by inducing production of monocistronic mRNA in the \^{I}²gal/CAT bicistronic reporter system}, url = {http://dx.doi.org/10.1261/rna.1557809}, volume = {15}, year = {2009} }

TY - JOUR ID - citeulike:5949614 L3 - citeulike-article-id:5949614 N2 - 10.1261/rna.1557809 X-chromosome linked inhibitor of apoptosis (XIAP) mRNA has been proposed to bear a stress-activated internal ribosome entry site (IRES) that stimulates translation under conditions that inhibit cap-dependent initiation. However, several reports have indicated that the strong activity of the XIAP IRES in certain bicistronic reporter assay systems stems from production of unintended monocistronic transcripts through splicing or cryptic promoter activity. Here we extend these findings by providing evidence that the XIAP IRES similarly provokes the production of monocistronic mRNA encompassing the 3′ cistron in the βgal/CAT bicistronic reporter plasmid that was originally used to identify and characterize this putative IRES, through cryptic promoter activity. IS - 11 JF - RNA EP - 1985 TI - The XIAP IRES activates 3′ cistron expression by inducing production of monocistronic mRNA in the βgal/CAT bicistronic reporter system VL - 15 SP - 1980 KW - ires KW - mrna KW - promoter_activity KW - x_chromosome AU - Saffran, Holly AU - Smiley, James PY - 2009/11// UR - http://dx.doi.org/10.1261/rna.1557809 ER -