Recombinant human antibodies specific for hepatitis C virus proteins. Export

@article{citeulike:1058013, abstract = {Human antibodies to hepatitis C virus core, NS4A and NS3 were cloned in a prokaryotic vector and expressed as soluble Fab fragments and as phage-displayed Fabs. The recombinant Fabs were shown to be a suitable tool for immunohistochemistry, since they recognize the cognate antigen expressed in mammalian cells. The nucleotide sequence of the cDNA for the variable domains of these antibodies was determined and the V-gene usage was derived. On the basis of the deduced amino acid sequence, a structural model of the V domains of the Fabs was constructed.}, address = {Istituto di Ricerche di Biologia Molecolare P. Angeletti (IRBM), Pomezia, Roma, Italy.}, author = {Esposito, G. and Morea, V. and Scarselli, E. and Cerino, A. and Mondelli, M. U. and La Monica, N. and Traboni, C.}, citeulike-article-id = {1058013}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9349306}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9349306}, issn = {0304-8608}, journal = {Arch Virol}, keywords = {antibody, hcv, hepatitis}, number = {3}, pages = {601--610}, posted-at = {2007-01-21 08:39:53}, priority = {2}, title = {Recombinant human antibodies specific for hepatitis C virus proteins.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9349306}, volume = {142}, year = {1997} }

TY - JOUR ID - citeulike:1058013 L3 - citeulike-article-id:1058013 N2 - Human antibodies to hepatitis C virus core, NS4A and NS3 were cloned in a prokaryotic vector and expressed as soluble Fab fragments and as phage-displayed Fabs. The recombinant Fabs were shown to be a suitable tool for immunohistochemistry, since they recognize the cognate antigen expressed in mammalian cells. The nucleotide sequence of the cDNA for the variable domains of these antibodies was determined and the V-gene usage was derived. On the basis of the deduced amino acid sequence, a structural model of the V domains of the Fabs was constructed. IS - 3 JF - Arch Virol SN - 0304-8608 AD - Istituto di Ricerche di Biologia Molecolare P. Angeletti (IRBM), Pomezia, Roma, Italy. EP - 610 TI - Recombinant human antibodies specific for hepatitis C virus proteins. VL - 142 SP - 601 KW - antibody KW - hcv KW - hepatitis AU - Esposito, G AU - Morea, V AU - Scarselli, E AU - Cerino, A AU - Mondelli, MU AU - La Monica, N AU - Traboni, C PY - 1997/// UR - http://view.ncbi.nlm.nih.gov/pubmed/9349306 ER -