Vbeta18.1(+) and V(alpha)2.3(+) T-cell subsets are associated with house dust mite allergy in human subjects. Export

@article{citeulike:2325310, abstract = {BACKGROUND: The recognition of allergenic peptides by T cells through their T-cell receptor (TCR) represents a crucial step in the initiation of an allergen-specific immune response. In parallel to the superantigen-driven restricted expansion of Vbeta subsets in autoimmune and infectious diseases, reports in animals and human subjects have shown a similar capacity of classical antigens. OBJECTIVE: The study was performed to analyze the V(alpha)/Vbeta expression in house dust mite (HDM) allergy. METHODS: The TCR repertoire of 15 subjects with HDM allergy, 22 atopic subjects without HDM allergy, and 19 nonatopic individuals, members of 2 extended and 4 nuclear families, was determined. By using flow cytometry, the expression of 22 Vbeta and 3 V(alpha) elements was analyzed in vivo and after in vitro allergen stimulation. RESULTS: In comparison with nonatopic and atopic individuals without HDM allergy, freshly isolated PBMCs of individuals with HDM allergy showed a significantly higher frequency of Vbeta18(+) and V(alpha)2.3(+) T cells. Although members of all 3 groups had a similar lymphocyte proliferation response after in vitro stimulation with Der p 1 or Der p 1 peptide(101-131), a significant expansion of Vbeta18(+) and V(alpha)2.3(+) T cells in vitro occurred only in individuals with HDM allergy. Moreover, the degree of expansion correlated with the levels of allergen-specific IgE antibodies. No expansion of Vbeta18(+) and V(alpha)2.3(+) was observed after mitogen stimulation with PHA, indicating allergen specificity of the response. CONCLUSION: Our results strongly suggest restricted TCR V(alpha)/Vbeta gene use in HDM allergy and might be a step toward TCR-based immunotherapy.}, address = {Institute of Clinical Chemistry and Biochemistry, Berlin, Germany.}, author = {Kircher, M. F. and Haeusler, T. and Nickel, R. and Lamb, J. R. and Renz, H. and Beyer, K.}, citeulike-article-id = {2325310}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/11898001}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=11898001}, issn = {0091-6749}, journal = {J Allergy Clin Immunol}, keywords = {asthma, usage, vgene}, month = {March}, number = {3}, pages = {517--523}, posted-at = {2008-02-03 10:18:50}, priority = {2}, title = {Vbeta18.1(+) and V(alpha)2.3(+) T-cell subsets are associated with house dust mite allergy in human subjects.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11898001}, volume = {109}, year = {2002} }

TY - JOUR ID - citeulike:2325310 L3 - citeulike-article-id:2325310 N2 - BACKGROUND: The recognition of allergenic peptides by T cells through their T-cell receptor (TCR) represents a crucial step in the initiation of an allergen-specific immune response. In parallel to the superantigen-driven restricted expansion of Vbeta subsets in autoimmune and infectious diseases, reports in animals and human subjects have shown a similar capacity of classical antigens. OBJECTIVE: The study was performed to analyze the V(alpha)/Vbeta expression in house dust mite (HDM) allergy. METHODS: The TCR repertoire of 15 subjects with HDM allergy, 22 atopic subjects without HDM allergy, and 19 nonatopic individuals, members of 2 extended and 4 nuclear families, was determined. By using flow cytometry, the expression of 22 Vbeta and 3 V(alpha) elements was analyzed in vivo and after in vitro allergen stimulation. RESULTS: In comparison with nonatopic and atopic individuals without HDM allergy, freshly isolated PBMCs of individuals with HDM allergy showed a significantly higher frequency of Vbeta18(+) and V(alpha)2.3(+) T cells. Although members of all 3 groups had a similar lymphocyte proliferation response after in vitro stimulation with Der p 1 or Der p 1 peptide(101-131), a significant expansion of Vbeta18(+) and V(alpha)2.3(+) T cells in vitro occurred only in individuals with HDM allergy. Moreover, the degree of expansion correlated with the levels of allergen-specific IgE antibodies. No expansion of Vbeta18(+) and V(alpha)2.3(+) was observed after mitogen stimulation with PHA, indicating allergen specificity of the response. CONCLUSION: Our results strongly suggest restricted TCR V(alpha)/Vbeta gene use in HDM allergy and might be a step toward TCR-based immunotherapy. IS - 3 JF - J Allergy Clin Immunol SN - 0091-6749 AD - Institute of Clinical Chemistry and Biochemistry, Berlin, Germany. EP - 523 TI - Vbeta18.1(+) and V(alpha)2.3(+) T-cell subsets are associated with house dust mite allergy in human subjects. VL - 109 SP - 517 KW - asthma KW - usage KW - vgene AU - Kircher, MF AU - Haeusler, T AU - Nickel, R AU - Lamb, JR AU - Renz, H AU - Beyer, K PY - 2002/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/11898001 ER -