Atypical VH-D-JH rearrangements in newborn autoimmune MRL mice. Export

@article{citeulike:623920, abstract = {Antinuclear Abs are the hallmark of the autoimmune disease systemic lupus erythematosus (SLE). The ability of self reactive autoantibodies to bind to DNA and nucleosomes is partly conferred by an increased number of arginine and asparagine residues in the heavy chain third complementarity determining region. This increased content of cationic residues is primarily the result of unusual VH-D-JH rearrangements, which include D-D fusions and D gene inversions. While self Ag-driven clonal expansion is a major contributor to the production of antinuclear Abs in lupus, we explore in this study the hypothesis that newly emerging B cells from autoimmune mice display more frequently these unusual VH-D-JH rearrangements. To this end, libraries of PCR-generated VH-D-JH junctions from MRL and C3H newborn livers were analyzed. When compared with the C3H controls, D and JH gene usage in MRL junctions suggests a greater frequency of secondary D-JH rearrangements in this strain. Furthermore, B cells from the autoimmune-prone MRL mice have significantly increased numbers of atypical VH-D-JH rearrangements (D-D fusions and D inversions). Therefore, B cells from MRL mice manifest intrinsic defects that could confer an increased propensity to produce unusual VH-D-JH rearrangements early in ontogeny.}, address = {Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA.}, author = {Klonowski, K. D. and Primiano, L. L. and Monestier, M.}, citeulike-article-id = {623920}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9973414}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9973414}, day = {1}, issn = {0022-1767}, journal = {J Immunol}, keywords = {1-4, anti-dna, ref, sle-}, month = {February}, number = {3}, pages = {1566--1572}, posted-at = {2006-05-11 22:29:47}, priority = {2}, title = {Atypical VH-D-JH rearrangements in newborn autoimmune MRL mice.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9973414}, volume = {162}, year = {1999} }

TY - JOUR ID - citeulike:623920 L3 - citeulike-article-id:623920 N2 - Antinuclear Abs are the hallmark of the autoimmune disease systemic lupus erythematosus (SLE). The ability of self reactive autoantibodies to bind to DNA and nucleosomes is partly conferred by an increased number of arginine and asparagine residues in the heavy chain third complementarity determining region. This increased content of cationic residues is primarily the result of unusual VH-D-JH rearrangements, which include D-D fusions and D gene inversions. While self Ag-driven clonal expansion is a major contributor to the production of antinuclear Abs in lupus, we explore in this study the hypothesis that newly emerging B cells from autoimmune mice display more frequently these unusual VH-D-JH rearrangements. To this end, libraries of PCR-generated VH-D-JH junctions from MRL and C3H newborn livers were analyzed. When compared with the C3H controls, D and JH gene usage in MRL junctions suggests a greater frequency of secondary D-JH rearrangements in this strain. Furthermore, B cells from the autoimmune-prone MRL mice have significantly increased numbers of atypical VH-D-JH rearrangements (D-D fusions and D inversions). Therefore, B cells from MRL mice manifest intrinsic defects that could confer an increased propensity to produce unusual VH-D-JH rearrangements early in ontogeny. IS - 3 JF - J Immunol SN - 0022-1767 AD - Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA. EP - 1572 TI - Atypical VH-D-JH rearrangements in newborn autoimmune MRL mice. VL - 162 SP - 1566 KW - 1-4 KW - anti-dna KW - ref KW - sle- AU - Klonowski, KD AU - Primiano, LL AU - Monestier, M PY - 1999/02/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9973414 ER -