Anti-DNA antibodies: aspects of structure and pathogenicity. Export

@article{citeulike:668866, abstract = {Anti-DNA antibodies contribute to the pathology of systemic lupus erythematosus. Their depositon in tissue lesions could result from localization of preformed immune complexes of antibodies with DNA or nucleosomes, or from cross-reaction of anti-DNA antibodies directly with tissue proteins. Structural analyses contribute to understanding their pathogenic potential. Primary structures of lupus immunoglobulin G double-stranded DNA-binding autoantibodies are determined by immunoglobulin genes with mutated variable region segments, indicative of selection by immunizing antigen. Arginine, lysine and asparagine residues in complementarity-determining region favor DNA binding. Heavy-chain variable regions make major contributions to DNA binding; affinity and specificity of binding are modulated or can be abrogated by the light-chain variable domain. Crytallographic structure is known for a few antibody-DNA complexes and several ligand-free Fab fragments. Computer modeling supplements this limited information. Structural information of lupus antibody interactions with both DNA and cross-reacting molecules will support use of ligands to inhibit tissue deposition of the antibodies and prevent lesion formation in lupus.}, address = {Laboratory of Immunology, Institute for Medical Science, Ajou University School of Medicine, Suwon 442-721, Korea. jangyj@madang.ajou.ac.kr}, author = {Jang, Y. J. and Stollar, B. D.}, citeulike-article-id = {668866}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12678496}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12678496}, issn = {1420-682X}, journal = {Cell Mol Life Sci}, keywords = {anti-dna}, month = {February}, number = {2}, pages = {309--320}, posted-at = {2006-05-24 20:07:15}, priority = {2}, title = {Anti-DNA antibodies: aspects of structure and pathogenicity.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12678496}, volume = {60}, year = {2003} }

TY - JOUR ID - citeulike:668866 L3 - citeulike-article-id:668866 N2 - Anti-DNA antibodies contribute to the pathology of systemic lupus erythematosus. Their depositon in tissue lesions could result from localization of preformed immune complexes of antibodies with DNA or nucleosomes, or from cross-reaction of anti-DNA antibodies directly with tissue proteins. Structural analyses contribute to understanding their pathogenic potential. Primary structures of lupus immunoglobulin G double-stranded DNA-binding autoantibodies are determined by immunoglobulin genes with mutated variable region segments, indicative of selection by immunizing antigen. Arginine, lysine and asparagine residues in complementarity-determining region favor DNA binding. Heavy-chain variable regions make major contributions to DNA binding; affinity and specificity of binding are modulated or can be abrogated by the light-chain variable domain. Crytallographic structure is known for a few antibody-DNA complexes and several ligand-free Fab fragments. Computer modeling supplements this limited information. Structural information of lupus antibody interactions with both DNA and cross-reacting molecules will support use of ligands to inhibit tissue deposition of the antibodies and prevent lesion formation in lupus. IS - 2 JF - Cell Mol Life Sci SN - 1420-682X AD - Laboratory of Immunology, Institute for Medical Science, Ajou University School of Medicine, Suwon 442-721, Korea. jangyj@madang.ajou.ac.kr EP - 320 TI - Anti-DNA antibodies: aspects of structure and pathogenicity. VL - 60 SP - 309 KW - anti-dna AU - Jang, YJ AU - Stollar, BD PY - 2003/02// UR - http://view.ncbi.nlm.nih.gov/pubmed/12678496 ER -