A single tube RT-PCR assay for the detection of mosquito-borne flaviviruses. Export

@article{citeulike:672793, abstract = {Mosquito-borne flaviviruses include several important agents of human disease and have provided striking examples of emerging infections. In this study we present the design and validation of a single tube RT-PCR assay using a pair of consensus primers for the detection of mosquito-borne flaviviruses. Sequencing of the amplicons permits the species identification. The assay was validated using RNA from the yellow fever virus vaccine strain and from representative strains of dengue viruses 1, 2, 3 and 4, West Nile virus, Kunjin virus (a clade of West Nile virus), and St. Louis encephalitis virus.}, address = {From the Metabolism Research Program, Hospital for Sick Children, Toronto, Ont., Canada.}, author = {Ayers, M and Adachi, D and Johnson, G and Andonova, M and Drebot, M and Tellier, R}, citeulike-article-id = {672793}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.jviromet.2006.03.009}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16650488}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16650488}, doi = {10.1016/j.jviromet.2006.03.009}, issn = {0166-0934}, journal = {J Virol Methods}, keywords = {load, mosquito, viral}, month = {April}, posted-at = {2006-05-28 07:04:36}, priority = {2}, title = {A single tube RT-PCR assay for the detection of mosquito-borne flaviviruses.}, url = {http://dx.doi.org/10.1016/j.jviromet.2006.03.009}, year = {2006} }

TY - JOUR ID - citeulike:672793 L3 - citeulike-article-id:672793 N2 - Mosquito-borne flaviviruses include several important agents of human disease and have provided striking examples of emerging infections. In this study we present the design and validation of a single tube RT-PCR assay using a pair of consensus primers for the detection of mosquito-borne flaviviruses. Sequencing of the amplicons permits the species identification. The assay was validated using RNA from the yellow fever virus vaccine strain and from representative strains of dengue viruses 1, 2, 3 and 4, West Nile virus, Kunjin virus (a clade of West Nile virus), and St. Louis encephalitis virus. JF - J Virol Methods SN - 0166-0934 AD - From the Metabolism Research Program, Hospital for Sick Children, Toronto, Ont., Canada. TI - A single tube RT-PCR assay for the detection of mosquito-borne flaviviruses. KW - load KW - mosquito KW - viral AU - Ayers, M AU - Adachi, D AU - Johnson, G AU - Andonova, M AU - Drebot, M AU - Tellier, R PY - 2006/04/27/ UR - http://dx.doi.org/10.1016/j.jviromet.2006.03.009 ER -