Introduction of an single nucleodite polymorphism-based "Major Y-chromosome haplogroup typing kit" suitable for predicting the geographical origin of male lineages.

@article{citeulike:512256, abstract = {The European Consortium "High-throughput analysis of single nucleotide polymorphisms for the forensic identification of persons--SNPforID", has performed a selection of candidate Y-chromosome single nucleotide polymorphisms (SNPs) for making inferences on the geographic origin of an unknown sample. From more than 200 SNPs compiled in the phylogenetic tree published by the Y-Chromosome Consortium, and looking at the population studies previously published, a package of 29 SNPs has been selected for the identification of major population haplogroups. A "Major Y-chromosome haplogroup typing kit" has been developed, which allows the multiplex amplification of all 29 SNPs in a single reaction. Allele genotyping was performed with a single base extension reaction (minisequencing) detected by CE. The validation of the multiplex was performed in a total of 1126 unrelated males distributed among 12 worldwide populations. The approach takes advantage of the specific geographic distribution of the Y-chromosome haplogroups and demonstrates the utility of binary polymorphisms to infer the origin of a male lineage.}, address = {Institute of Legal Medicine, National Genotyping Center (CEGEN), University of Santiago de Compostela, Spain. brioniml@usc.es}, author = {Bri\'{o}n, M. and Sanchez, J. J. and Balogh, K. and Thacker, C. and Blanco-Verea, A. and Børsting, C. and Stradmann-Bellinghausen, B. and Bogus, M. and Syndercombe-Court, D. and Schneider, P. M. and Carracedo, A. and Morling, N. }, citeulike-article-id = {512256}, doi = {10.1002/elps.200500293}, issn = {0173-0835}, journal = {Electrophoresis}, keywords = {haplotyping, y-chromosome}, month = {December}, number = {23}, pages = {4411--4420}, posted-at = {2007-04-19 08:41:45}, priority = {2}, title = {Introduction of an single nucleodite polymorphism-based "Major Y-chromosome haplogroup typing kit" suitable for predicting the geographical origin of male lineages.}, url = {http://dx.doi.org/10.1002/elps.200500293}, volume = {26}, year = {2005} }

TY - JOUR ID - citeulike:512256 L3 - citeulike-article-id:512256 TI - Introduction of an single nucleodite polymorphism-based "Major Y-chromosome haplogroup typing kit" suitable for predicting the geographical origin of male lineages. JF - Electrophoresis VL - 26 IS - 23 SP - 4411 EP - 4420 AD - Institute of Legal Medicine, National Genotyping Center (CEGEN), University of Santiago de Compostela, Spain. brioniml@usc.es SN - 0173-0835 N2 - The European Consortium "High-throughput analysis of single nucleotide polymorphisms for the forensic identification of persons--SNPforID", has performed a selection of candidate Y-chromosome single nucleotide polymorphisms (SNPs) for making inferences on the geographic origin of an unknown sample. From more than 200 SNPs compiled in the phylogenetic tree published by the Y-Chromosome Consortium, and looking at the population studies previously published, a package of 29 SNPs has been selected for the identification of major population haplogroups. A "Major Y-chromosome haplogroup typing kit" has been developed, which allows the multiplex amplification of all 29 SNPs in a single reaction. Allele genotyping was performed with a single base extension reaction (minisequencing) detected by CE. The validation of the multiplex was performed in a total of 1126 unrelated males distributed among 12 worldwide populations. The approach takes advantage of the specific geographic distribution of the Y-chromosome haplogroups and demonstrates the utility of binary polymorphisms to infer the origin of a male lineage. KW - haplotyping KW - y-chromosome AU - Brión, M AU - Sanchez, JJ AU - Balogh, K AU - Thacker, C AU - Blanco-Verea, A AU - Børsting, C AU - Stradmann-Bellinghausen, B AU - Bogus, M AU - Syndercombe-Court, D AU - Schneider, PM AU - Carracedo, A AU - Morling, N PY - 2005/12// UR - http://dx.doi.org/10.1002/elps.200500293 ER -