Mechanism controlling the extended lag period associated with vinyl chloride starvation in Nocardioides sp. strain JS614

@article{citeulike:1167364, abstract = {The extended lag period associated with vinyl chloride (VC) starvation in VC- and ethene-assimilating Nocardioides sp. strain JS614 was examined. The extended lag periods were variable (3–7 days), only associated with growth on VC or ethene, and were observed in VC- or ethene-grown cultures following 24 h carbon starvation and mid-exponential phase cultures grown on non-alkene carbon sources (e.g. acetate). Alkene monooxygenase (AkMO) and epoxyalkane:coenzyme M transferase (EaCoMT) are the initial enzymes of VC and ethene biodegradation in strain JS614. Reverse-transcription PCR confirmed that the AkMO gene etnC was expressed in response to epoxyethane, a metabolic intermediate of ethene biodegradation. Epoxyethane (0.5 mM) eliminated the extended lag period in both starved and mid-exponential phase cultures, suggesting that epoxyethane accumulation activates AkMO expression in strain JS614. AkMO activity in ethene-grown cultures was not detected after 6.7 h of carbon starvation, while 40\% of the initial EaCoMT activity remained after 24 h. Acetate eliminated the extended lag period in starved cultures but not in mid-exponential phase cultures suggesting that acetate reactivates extant AkMO in starved VC- or ethene-grown cultures. The imbalance between AkMO and EaCoMT activities during starvation likely contributes to the extended lag period by delaying epoxide accumulation and subsequent AkMO induction.}, author = {Mattes and Timothy and Coleman and Nicholas and Chuang and Adina and Rogers and Andrea and Spain and Jim and Gossett and James}, citeulike-article-id = {1167364}, doi = {http://dx.doi.org/10.1007/s00203-006-0189-2}, issn = {0302-8933}, journal = {Archives of Microbiology}, keywords = {biodegradation, for-the-review, inducers, integration, regulation, transcription}, month = {March}, number = {3}, pages = {217--226}, posted-at = {2008-07-21 17:24:47}, priority = {3}, publisher = {Springer}, title = {Mechanism controlling the extended lag period associated with vinyl chloride starvation in Nocardioides sp. strain JS614}, url = {http://dx.doi.org/10.1007/s00203-006-0189-2}, volume = {187}, year = {2007} }

TY - JOUR ID - citeulike:1167364 L3 - citeulike-article-id:1167364 TI - Mechanism controlling the extended lag period associated with vinyl chloride starvation in Nocardioides sp. strain JS614 JF - Archives of Microbiology VL - 187 IS - 3 SP - 217 EP - 226 PB - Springer SN - 0302-8933 N2 - The extended lag period associated with vinyl chloride (VC) starvation in VC- and ethene-assimilating Nocardioides sp. strain JS614 was examined. The extended lag periods were variable (3–7 days), only associated with growth on VC or ethene, and were observed in VC- or ethene-grown cultures following 24 h carbon starvation and mid-exponential phase cultures grown on non-alkene carbon sources (e.g. acetate). Alkene monooxygenase (AkMO) and epoxyalkane:coenzyme M transferase (EaCoMT) are the initial enzymes of VC and ethene biodegradation in strain JS614. Reverse-transcription PCR confirmed that the AkMO gene etnC was expressed in response to epoxyethane, a metabolic intermediate of ethene biodegradation. Epoxyethane (0.5 mM) eliminated the extended lag period in both starved and mid-exponential phase cultures, suggesting that epoxyethane accumulation activates AkMO expression in strain JS614. AkMO activity in ethene-grown cultures was not detected after 6.7 h of carbon starvation, while 40% of the initial EaCoMT activity remained after 24 h. Acetate eliminated the extended lag period in starved cultures but not in mid-exponential phase cultures suggesting that acetate reactivates extant AkMO in starved VC- or ethene-grown cultures. The imbalance between AkMO and EaCoMT activities during starvation likely contributes to the extended lag period by delaying epoxide accumulation and subsequent AkMO induction. KW - biodegradation KW - for-the-review KW - inducers KW - integration KW - regulation KW - transcription AU - Mattes AU - Timothy AU - Coleman AU - Nicholas AU - Chuang AU - Adina AU - Rogers AU - Andrea AU - Spain AU - Jim AU - Gossett AU - James PY - 2007/03// UR - http://dx.doi.org/10.1007/s00203-006-0189-2 ER -