Identification and analysis of a glutaryl-CoA dehydrogenase-encoding gene and its cognate transcriptional regulator from Azoarcus sp. CIB Export

@article{citeulike:2223776, abstract = {In this work, the gcdH gene from the denitrifying beta-proteobacterium Azoarcus sp. CIB was shown to encode a glutaryl-CoA dehydrogenase, which is essential for the anaerobic catabolism of many aromatic compounds and some alicyclic and dicarboxylic acids. The primary structure of the GcdH protein is highly conserved in many organisms. The divergently transcribed gcdR gene, encoding a LysR-type transcriptional regulator, accounts for the glutaconate/glutarate-specific activation of the Pg promoter driving expression of gcdH. The Azoarcus sp. CIBdgcdH mutant strain harbouring a disrupted gcdH gene was used as host to identify heterologous gcdH genes, such as that from Pseudomonas putida KT2440. Moreover, the expression of gcdH from P. putida can be efficiently controlled by the GcdR activator in Azoarcus sp. CIB, demonstrating the existence of cross-talk between GcdR regulators and gcdH promoters from members of different phylogenetic subgroups of proteobacteria.}, author = {Blazquez and Blas and Carmona and Manuel and Garcia and Luis, Jose and Diaz and Eduardo}, citeulike-article-id = {2223776}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1462-2920.2007.01468.x}, citeulike-linkout-1 = {http://www.ingentaconnect.com/content/bsc/emi/2008/00000010/00000002/art00017}, citeulike-linkout-2 = {http://www3.interscience.wiley.com/cgi-bin/abstract/119419923/ABSTRACT}, doi = {10.1111/j.1462-2920.2007.01468.x}, issn = {1462-2912}, journal = {Environmental Microbiology}, keywords = {biodegradation, for-the-review, lysr, regulation, transcription}, month = {February}, number = {2}, pages = {474--482}, posted-at = {2008-07-21 16:52:28}, priority = {3}, publisher = {Blackwell Publishing}, title = {Identification and analysis of a glutaryl-CoA dehydrogenase-encoding gene and its cognate transcriptional regulator from Azoarcus sp. CIB}, url = {http://dx.doi.org/10.1111/j.1462-2920.2007.01468.x}, volume = {10}, year = {2008} }

TY - JOUR ID - citeulike:2223776 L3 - citeulike-article-id:2223776 N2 - In this work, the gcdH gene from the denitrifying beta-proteobacterium Azoarcus sp. CIB was shown to encode a glutaryl-CoA dehydrogenase, which is essential for the anaerobic catabolism of many aromatic compounds and some alicyclic and dicarboxylic acids. The primary structure of the GcdH protein is highly conserved in many organisms. The divergently transcribed gcdR gene, encoding a LysR-type transcriptional regulator, accounts for the glutaconate/glutarate-specific activation of the Pg promoter driving expression of gcdH. The Azoarcus sp. CIBdgcdH mutant strain harbouring a disrupted gcdH gene was used as host to identify heterologous gcdH genes, such as that from Pseudomonas putida KT2440. Moreover, the expression of gcdH from P. putida can be efficiently controlled by the GcdR activator in Azoarcus sp. CIB, demonstrating the existence of cross-talk between GcdR regulators and gcdH promoters from members of different phylogenetic subgroups of proteobacteria. IS - 2 JF - Environmental Microbiology SN - 1462-2912 EP - 482 TI - Identification and analysis of a glutaryl-CoA dehydrogenase-encoding gene and its cognate transcriptional regulator from Azoarcus sp. CIB PB - Blackwell Publishing VL - 10 SP - 474 KW - biodegradation KW - for-the-review KW - lysr KW - regulation KW - transcription AU - Blazquez AU - Blas AU - Carmona AU - Manuel AU - Garcia AU - Luis, Jose AU - Diaz AU - Eduardo PY - 2008/02// UR - http://dx.doi.org/10.1111/j.1462-2920.2007.01468.x ER -