Selection of biocatalysts for chemical synthesis. Export

@article{citeulike:3024788, abstract = {To determine whether microbial chemosensors can be used to find new or better biocatalysts, we constructed Escherichia coli hosts that recognize the product of a biocatalytic conversion through the transcriptional activator NahR and respond by expression of a lacZ or tetA reporter gene. Equipped with a benzaldehyde dehydrogenase (XylC from Pseudomonas putida), the lacZ-based host responded to the oxidation of benzaldehyde and 2-hydroxybenzaldehyde to the corresponding benzoic acids by forming blue colonies, whereas XylC- cells did not. Similarly, the tetA-based host was able to grow under selective conditions only when equipped with XylC, enabling selection of biocatalytically active cells in inactive populations at frequencies as low as 10(-6).}, address = {Institute of Biotechnology, Eidgen\"{o}ssische Technische Hochschule H\"{o}nggerberg, HPT Building, Wolfgang-Pauli-Strasse 16, 8093 Z\"{u}rich, Switzerland.}, author = {van Sint Fiet, S. and van Beilen, J. B. and Witholt, B.}, citeulike-article-id = {3024788}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0504733102}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16446453}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16446453}, day = {7}, doi = {10.1073/pnas.0504733102}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {biodegradation, biosensors, for-the-review, regulation, transcription}, month = {February}, number = {6}, pages = {1693--1698}, posted-at = {2008-07-21 16:56:36}, priority = {3}, title = {Selection of biocatalysts for chemical synthesis.}, url = {http://dx.doi.org/10.1073/pnas.0504733102}, volume = {103}, year = {2006} }

TY - JOUR ID - citeulike:3024788 L3 - citeulike-article-id:3024788 N2 - To determine whether microbial chemosensors can be used to find new or better biocatalysts, we constructed Escherichia coli hosts that recognize the product of a biocatalytic conversion through the transcriptional activator NahR and respond by expression of a lacZ or tetA reporter gene. Equipped with a benzaldehyde dehydrogenase (XylC from Pseudomonas putida), the lacZ-based host responded to the oxidation of benzaldehyde and 2-hydroxybenzaldehyde to the corresponding benzoic acids by forming blue colonies, whereas XylC- cells did not. Similarly, the tetA-based host was able to grow under selective conditions only when equipped with XylC, enabling selection of biocatalytically active cells in inactive populations at frequencies as low as 10(-6). IS - 6 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 AD - Institute of Biotechnology, Eidgenössische Technische Hochschule Hönggerberg, HPT Building, Wolfgang-Pauli-Strasse 16, 8093 Zürich, Switzerland. EP - 1698 TI - Selection of biocatalysts for chemical synthesis. VL - 103 SP - 1693 KW - biodegradation KW - biosensors KW - for-the-review KW - regulation KW - transcription AU - van Sint Fiet, S AU - van Beilen, JB AU - Witholt, B PY - 2006/02/07/ UR - http://dx.doi.org/10.1073/pnas.0504733102 ER -