Functional profiling of the Saccharomyces cerevisiae genome.

@article{citeulike:479086, abstract = {Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically constructed a nearly complete collection of gene-deletion mutants (96\% of annotated open reading frames, or ORFs) of the yeast Saccharomyces cerevisiae. DNA sequences dubbed 'molecular bar codes' uniquely identify each strain, enabling their growth to be analysed in parallel and the fitness contribution of each gene to be quantitatively assessed by hybridization to high-density oligonucleotide arrays. We show that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment. Less than 7\% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal growth in four of the tested conditions. Our results validate the yeast gene-deletion collection as a valuable resource for functional genomics.}, address = {Stanford Genome Technology Center, Stanford University, Palo Alto, California 94304, USA.}, author = {Giaever, G. and Chu, A. M. and Ni, L. and Connelly, C. and Riles, L. and V\'{e}ronneau, S. and Dow, S. and Lucau-Danila, A. and Anderson, K. and Andr\'{e}, B. and Arkin, A. P. and Astromoff, A. and El-Bakkoury, M. and Bangham, R. and Benito, R. and Brachat, S. and Campanaro, S. and Curtiss, M. and Davis, K. and Deutschbauer, A. and Entian, K. D. and Flaherty, P. and Foury, F. and Garfinkel, D. J. and Gerstein, M. and Gotte, D. and G\"{u}ldener, U. and Hegemann, J. H. and Hempel, S. and Herman, Z. and Jaramillo, D. F. and Kelly, D. E. and Kelly, S. L. and K\"{o}tter, P. and LaBonte, D. and Lamb, D. C. and Lan, N. and Liang, H. and Liao, H. and Liu, L. and Luo, C. and Lussier, M. and Mao, R. and Menard, P. and Ooi, S. L. and Revuelta, J. L. and Roberts, C. J. and Rose, M. and Ross-Macdonald, P. and Scherens, B. and Schimmack, G. and Shafer, B. and Shoemaker, D. D. and Sookhai-Mahadeo, S. and Storms, R. K. and Strathern, J. N. and Valle, G. and Voet, M. and Volckaert, G. and Wang, C. Y. and Ward, T. R. and Wilhelmy, J. and Winzeler, E. A. and Yang, Y. and Yen, G. and Youngman, E. and Yu, K. and Bussey, H. and Boeke, J. D. and Snyder, M. and Philippsen, P. and Davis, R. W. and Johnston, M. }, citeulike-article-id = {479086}, doi = {http://dx.doi.org/10.1038/nature00935}, issn = {0028-0836}, journal = {Nature}, keywords = {deletion, genomics, ht, mutant, yeast}, month = {July}, number = {6896}, pages = {387--391}, posted-at = {2007-08-10 09:05:47}, priority = {3}, title = {Functional profiling of the Saccharomyces cerevisiae genome.}, url = {http://dx.doi.org/10.1038/nature00935}, volume = {418}, year = {2002} }

TY - JOUR ID - citeulike:479086 L3 - citeulike-article-id:479086 TI - Functional profiling of the Saccharomyces cerevisiae genome. JF - Nature VL - 418 IS - 6896 SP - 387 EP - 391 AD - Stanford Genome Technology Center, Stanford University, Palo Alto, California 94304, USA. SN - 0028-0836 N2 - Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically constructed a nearly complete collection of gene-deletion mutants (96% of annotated open reading frames, or ORFs) of the yeast Saccharomyces cerevisiae. DNA sequences dubbed 'molecular bar codes' uniquely identify each strain, enabling their growth to be analysed in parallel and the fitness contribution of each gene to be quantitatively assessed by hybridization to high-density oligonucleotide arrays. We show that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment. Less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal growth in four of the tested conditions. Our results validate the yeast gene-deletion collection as a valuable resource for functional genomics. KW - deletion KW - genomics KW - ht KW - mutant KW - yeast AU - Giaever, G AU - Chu, AM AU - Ni, L AU - Connelly, C AU - Riles, L AU - Véronneau, S AU - Dow, S AU - Lucau-Danila, A AU - Anderson, K AU - André, B AU - Arkin, AP AU - Astromoff, A AU - El-Bakkoury, M AU - Bangham, R AU - Benito, R AU - Brachat, S AU - Campanaro, S AU - Curtiss, M AU - Davis, K AU - Deutschbauer, A AU - Entian, KD AU - Flaherty, P AU - Foury, F AU - Garfinkel, DJ AU - Gerstein, M AU - Gotte, D AU - Güldener, U AU - Hegemann, JH AU - Hempel, S AU - Herman, Z AU - Jaramillo, DF AU - Kelly, DE AU - Kelly, SL AU - Kötter, P AU - LaBonte, D AU - Lamb, DC AU - Lan, N AU - Liang, H AU - Liao, H AU - Liu, L AU - Luo, C AU - Lussier, M AU - Mao, R AU - Menard, P AU - Ooi, SL AU - Revuelta, JL AU - Roberts, CJ AU - Rose, M AU - Ross-Macdonald, P AU - Scherens, B AU - Schimmack, G AU - Shafer, B AU - Shoemaker, DD AU - Sookhai-Mahadeo, S AU - Storms, RK AU - Strathern, JN AU - Valle, G AU - Voet, M AU - Volckaert, G AU - Wang, CY AU - Ward, TR AU - Wilhelmy, J AU - Winzeler, EA AU - Yang, Y AU - Yen, G AU - Youngman, E AU - Yu, K AU - Bussey, H AU - Boeke, JD AU - Snyder, M AU - Philippsen, P AU - Davis, RW AU - Johnston, M PY - 2002/07/25/ UR - http://dx.doi.org/10.1038/nature00935 ER -