Two-photon laser scanning fluorescence microscopy Export

@article{citeulike:1911645, abstract = {Molecular excitation by the simultaneous absorption of two photons provides intrinsic three-dimensional resolution in laser scanning fluorescence microscopy. The excitation of fluorophores having single-photon absorption in the ultraviolet with a stream of strongly focused subpicosecond pulses of red laser light has made possible fluorescence images of living cells and other microscopic objects. The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation. This technique also provides unprecedented capabilities for three-dimensional, spatially resolved photochemistry, particularly photolytic release of caged effector molecules. 10.1126/science.2321027}, author = {Denk, W. and Strickler, J. H. and Webb, W. W.}, citeulike-article-id = {1911645}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.2321027}, citeulike-linkout-1 = {http://www.sciencemag.org/content/248/4951/73.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/248/4951/73.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/248/4951/73}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/2321027}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=2321027}, day = {6}, doi = {10.1126/science.2321027}, journal = {Science}, keywords = {3d, microscopy, two-photon}, month = {April}, number = {4951}, pages = {73--76}, posted-at = {2009-11-05 06:13:01}, priority = {2}, title = {Two-photon laser scanning fluorescence microscopy}, url = {http://dx.doi.org/10.1126/science.2321027}, volume = {248}, year = {1990} }

TY - JOUR ID - citeulike:1911645 L3 - citeulike-article-id:1911645 N2 - Molecular excitation by the simultaneous absorption of two photons provides intrinsic three-dimensional resolution in laser scanning fluorescence microscopy. The excitation of fluorophores having single-photon absorption in the ultraviolet with a stream of strongly focused subpicosecond pulses of red laser light has made possible fluorescence images of living cells and other microscopic objects. The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation. This technique also provides unprecedented capabilities for three-dimensional, spatially resolved photochemistry, particularly photolytic release of caged effector molecules. 10.1126/science.2321027 IS - 4951 JF - Science EP - 76 TI - Two-photon laser scanning fluorescence microscopy VL - 248 SP - 73 KW - 3d KW - microscopy KW - two-photon AU - Denk, W AU - Strickler, JH AU - Webb, WW PY - 1990/04/06/ UR - http://dx.doi.org/10.1126/science.2321027 ER -