STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins Export

@article{citeulike:5761030, abstract = {We demonstrate the effectiveness of a genetically encoded malachite green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an intracellular FAP-actin fusion. Structures with widths of 110−122 nm were observed. Depletion data, however, suggest a resolution of 70 nm with the given instrument.}, author = {Fitzpatrick, James A. J. and Yan, Qi and Sieber, Jochen J. and Dyba, Marcus and Schwarz, Ulf and Szent-Gyorgyi, Chris and Woolford, Carol A. and Berget, Peter B. and Waggoner, Alan S. and Bruchez, Marcel P.}, citeulike-article-id = {5761030}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/bc900249e}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/bc900249e}, doi = {10.1021/bc900249e}, journal = {Bioconjugate Chemistry}, keywords = {aptamers, bioconjugation, fluorogenic, readout, super-resolution}, number = {0}, posted-at = {2009-09-09 22:10:40}, priority = {2}, title = {STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins}, url = {http://dx.doi.org/10.1021/bc900249e}, volume = {0}, year = {0000} }

TY - JOUR ID - citeulike:5761030 L3 - citeulike-article-id:5761030 N2 - We demonstrate the effectiveness of a genetically encoded malachite green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an intracellular FAP-actin fusion. Structures with widths of 110−122 nm were observed. Depletion data, however, suggest a resolution of 70 nm with the given instrument. IS - 0 JF - Bioconjugate Chemistry TI - STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins VL - 0 KW - aptamers KW - bioconjugation KW - fluorogenic KW - readout KW - super-resolution AU - Fitzpatrick, James AU - Yan, Qi AU - Sieber, Jochen AU - Dyba, Marcus AU - Schwarz, Ulf AU - Szent-Gyorgyi, Chris AU - Woolford, Carol AU - Berget, Peter AU - Waggoner, Alan AU - Bruchez, Marcel PY - 2000/// UR - http://dx.doi.org/10.1021/bc900249e ER -