Azido Push−Pull Fluorogens Photoactivate to Produce Bright Fluorescent Labels† Export

@article{citeulike:6016756, abstract = {Dark azido push−pull chromophores have the ability to be photoactivated to produce bright fluorescent labels suitable for single-molecule imaging. Upon illumination, the aryl azide functionality in the fluorogens participates in a photochemical conversion to an aryl amine, thus restoring charge-transfer absorption and fluorescence. Previously, we reported that one compound, DCDHF-V-P-azide, was photoactivatable. Here, we demonstrate that the azide-to-amine photoactivation process is generally applicable to a variety of push−pull chromophores, and we characterize the photophysical parameters including photoconversion quantum yield, photostability, and turn-on ratio. Azido push−pull fluorogens provide a new class of photoactivatable single-molecule probes for fluorescent labeling and super-resolution microscopy. Lastly, we demonstrate that photoactivated push−pull dyes can insert into bonds of nearby biomolecules, simultaneously forming a covalent bond and becoming fluorescent (fluorogenic photoaffinity labeling).}, author = {Lord, Samuel J. and Lee, Hsiao-lu D. and Samuel, Reichel and Weber, Ryan and Liu, Na and Conley, Nicholas R. and Thompson, Michael A. and Twieg, Robert J. and Moerner, W. E.}, citeulike-article-id = {6016756}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/jp907080r}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/jp907080r}, doi = {10.1021/jp907080r}, journal = {The Journal of Physical Chemistry B}, keywords = {charge-transfer, click-chemistry, dcdhf, fluorogenic, fluorophores, photochemistry, photophysics, photoswitchable}, number = {0}, posted-at = {2009-10-27 20:09:54}, priority = {2}, title = {Azido Push−Pull Fluorogens Photoactivate to Produce Bright Fluorescent Labels{\dag}}, url = {http://dx.doi.org/10.1021/jp907080r}, volume = {0}, year = {0000} }

TY - JOUR ID - citeulike:6016756 L3 - citeulike-article-id:6016756 N2 - Dark azido push−pull chromophores have the ability to be photoactivated to produce bright fluorescent labels suitable for single-molecule imaging. Upon illumination, the aryl azide functionality in the fluorogens participates in a photochemical conversion to an aryl amine, thus restoring charge-transfer absorption and fluorescence. Previously, we reported that one compound, DCDHF-V-P-azide, was photoactivatable. Here, we demonstrate that the azide-to-amine photoactivation process is generally applicable to a variety of push−pull chromophores, and we characterize the photophysical parameters including photoconversion quantum yield, photostability, and turn-on ratio. Azido push−pull fluorogens provide a new class of photoactivatable single-molecule probes for fluorescent labeling and super-resolution microscopy. Lastly, we demonstrate that photoactivated push−pull dyes can insert into bonds of nearby biomolecules, simultaneously forming a covalent bond and becoming fluorescent (fluorogenic photoaffinity labeling). IS - 0 JF - The Journal of Physical Chemistry B TI - Azido Push−Pull Fluorogens Photoactivate to Produce Bright Fluorescent Labels† VL - 0 KW - charge-transfer KW - click-chemistry KW - dcdhf KW - fluorogenic KW - fluorophores KW - photochemistry KW - photophysics KW - photoswitchable AU - Lord, Samuel AU - Lee, Hsiao-lu AU - Samuel, Reichel AU - Weber, Ryan AU - Liu, Na AU - Conley, Nicholas AU - Thompson, Michael AU - Twieg, Robert AU - Moerner, WE PY - 2000/// UR - http://dx.doi.org/10.1021/jp907080r ER -