Photophysical Properties of a New DyLight 594 Dye Export

@article{citeulike:6077103, abstract = {We describe spectral properties of novel fluorescence probe DyLight(TM) 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50\%. The new DyLight dye also shows slightly longer lifetime and photostability. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make this fluorophore attractive as a fluorescence probe in biochemical/biological studies involving fluorescence methods.}, author = {Sarkar, Pabak and Sridharan, Savitha and Luchowski, Rafal and Desai, Surbhi and Dworecki, Boguslawa and Nlend, Marie and Gryczynski, Zygmunt and Gryczynski, Ignacy}, citeulike-article-id = {6077103}, citeulike-linkout-0 = {http://www.sciencedirect.com/science/article/B6TH0-4XM6K9G-1/2/7d4bd2902fc7f4d2d2ec24c78005789c}, citeulike-linkout-1 = {http://dx.doi.org/DOI:%2010.1016/j.jphotobiol.2009.10.005}, doi = {DOI:%2010.1016/j.jphotobiol.2009.10.005}, journal = {Journal of Photochemistry and Photobiology B: Biology}, keywords = {fluorophores, photophysics}, posted-at = {2009-11-05 22:20:24}, priority = {2}, title = {Photophysical Properties of a New DyLight 594 Dye}, url = {http://dx.doi.org/DOI:%2010.1016/j.jphotobiol.2009.10.005}, volume = {In Press, Accepted Manuscript}, year = {2009} }

TY - JOUR ID - citeulike:6077103 L3 - citeulike-article-id:6077103 N2 - We describe spectral properties of novel fluorescence probe DyLight(TM) 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50%. The new DyLight dye also shows slightly longer lifetime and photostability. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make this fluorophore attractive as a fluorescence probe in biochemical/biological studies involving fluorescence methods. JF - Journal of Photochemistry and Photobiology B: Biology TI - Photophysical Properties of a New DyLight 594 Dye VL - In Press, Accepted Manuscript KW - fluorophores KW - photophysics AU - Sarkar, Pabak AU - Sridharan, Savitha AU - Luchowski, Rafal AU - Desai, Surbhi AU - Dworecki, Boguslawa AU - Nlend, Marie AU - Gryczynski, Zygmunt AU - Gryczynski, Ignacy PY - 2009/// UR - http://dx.doi.org/DOI:%2010.1016/j.jphotobiol.2009.10.005 ER -