Movement and equipositioning of plasmids by ParA filament disassembly Export

@article{citeulike:6100186, abstract = {10.1073/pnas.0908347106 Bacterial plasmids encode partitioning () loci that confer stable plasmid inheritance. We showed previously that, in the presence of ParB and encoded by the locus of plasmid pB171, ParA formed cytoskeletal-like structures that dynamically relocated over the nucleoid. Simultaneously, the locus distributed plasmids regularly over the nucleoid. We show here that the dynamic ParA patterns are not simple oscillations. Rather, ParA nucleates and polymerizes in between plasmids. When a ParA assembly reaches a plasmid, the assembly reaction reverses into disassembly. Strikingly, plasmids consistently migrate behind disassembling ParA cytoskeletal structures, suggesting that ParA filaments pull plasmids by depolymerization. The perpetual cycles of ParA assembly and disassembly result in continuous relocation of plasmids, which, on time averaging, results in equidistribution of the plasmids. Mathematical modeling of ParA and plasmid dynamics support these interpretations. Mutational analysis supports a molecular mechanism in which the ParB/ complex controls ParA filament depolymerization.}, author = {Ringgaard, Simon and van Zon, Jeroen and Howard, Martin and Gerdes, Kenn}, citeulike-article-id = {6100186}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0908347106}, citeulike-linkout-1 = {http://www.pnas.org/content/0908347106v1/.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/0908347106v1/.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19906997}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19906997}, day = {17}, doi = {10.1073/pnas.0908347106}, journal = {Proceedings of the National Academy of Sciences}, keywords = {cell-biology, cytoskeleton, proteins}, month = {November}, number = {46}, pages = {19369--19374}, posted-at = {2009-11-11 22:29:12}, priority = {2}, title = {Movement and equipositioning of plasmids by ParA filament disassembly}, url = {http://dx.doi.org/10.1073/pnas.0908347106}, volume = {106}, year = {2009} }

TY - JOUR ID - citeulike:6100186 L3 - citeulike-article-id:6100186 N2 - 10.1073/pnas.0908347106 Bacterial plasmids encode partitioning () loci that confer stable plasmid inheritance. We showed previously that, in the presence of ParB and encoded by the locus of plasmid pB171, ParA formed cytoskeletal-like structures that dynamically relocated over the nucleoid. Simultaneously, the locus distributed plasmids regularly over the nucleoid. We show here that the dynamic ParA patterns are not simple oscillations. Rather, ParA nucleates and polymerizes in between plasmids. When a ParA assembly reaches a plasmid, the assembly reaction reverses into disassembly. Strikingly, plasmids consistently migrate behind disassembling ParA cytoskeletal structures, suggesting that ParA filaments pull plasmids by depolymerization. The perpetual cycles of ParA assembly and disassembly result in continuous relocation of plasmids, which, on time averaging, results in equidistribution of the plasmids. Mathematical modeling of ParA and plasmid dynamics support these interpretations. Mutational analysis supports a molecular mechanism in which the ParB/ complex controls ParA filament depolymerization. IS - 46 JF - Proceedings of the National Academy of Sciences EP - 19374 TI - Movement and equipositioning of plasmids by ParA filament disassembly VL - 106 SP - 19369 KW - cell-biology KW - cytoskeleton KW - proteins AU - Ringgaard, Simon AU - van Zon, Jeroen AU - Howard, Martin AU - Gerdes, Kenn PY - 2009/11/17/ UR - http://dx.doi.org/10.1073/pnas.0908347106 ER -