Poised Polymerases: On Your Mark...Get Set...Go! Export

@article{citeulike:2678194, abstract = {Recent global analyses have determined that many Drosophila and human genes have engaged polymerase molecules trapped immediately downstream of promoters. These results strongly implicate RNA polymerase II elongation control as a major regulator of differentiation and development.}, author = {Price, David H.}, citeulike-article-id = {2678194}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.molcel.2008.03.001}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WSR-4S80780-4/1/41e82a8ec116f1b5d17dce226ed5e39b}, comment = {A great review of promoter proximal elongating polymerases (PPEP) aka "poised polymerases". A lot devoted to the 2007 Muse et al. and Zeitlinger et al. studies showing how PPEP is all over the genome. Price says, "Although it is experimentally difficult to address, it is important to determine the duration of engagement of the poised polymerases"...this made me realize something. We are working for the ability to detect Pol II molecules on individual chromatin fibers, based on unzipping single fibers with optical tweezers. If successful, we can measure the DISTANCE between the PPEP polymerase (n=0), and the closest downstream polymerase (n=1), and the next closest(n=2). Analysis of these distances would give information on the PPEP release kinetics, I think...}, day = {11}, doi = {10.1016/j.molcel.2008.03.001}, journal = {Molecular Cell}, keywords = {elongation, paused, polymerase, ppep, review, rnap, transcription}, month = {April}, number = {1}, pages = {7--10}, posted-at = {2009-02-09 04:23:58}, priority = {0}, title = {Poised Polymerases: On Your Mark...Get Set...Go!}, url = {http://dx.doi.org/10.1016/j.molcel.2008.03.001}, volume = {30}, year = {2008} }

TY - JOUR ID - citeulike:2678194 L3 - citeulike-article-id:2678194 N2 - Recent global analyses have determined that many Drosophila and human genes have engaged polymerase molecules trapped immediately downstream of promoters. These results strongly implicate RNA polymerase II elongation control as a major regulator of differentiation and development. IS - 1 JF - Molecular Cell EP - 10 TI - Poised Polymerases: On Your Mark...Get Set...Go! VL - 30 SP - 7 KW - elongation KW - paused KW - polymerase KW - ppep KW - review KW - rnap KW - transcription N1 - A great review of promoter proximal elongating polymerases (PPEP) aka "poised polymerases". A lot devoted to the 2007 Muse et al. and Zeitlinger et al. studies showing how PPEP is all over the genome. Price says, "Although it is experimentally difficult to address, it is important to determine the duration of engagement of the poised polymerases"...this made me realize something. We are working for the ability to detect Pol II molecules on individual chromatin fibers, based on unzipping single fibers with optical tweezers. If successful, we can measure the DISTANCE between the PPEP polymerase (n=0), and the closest downstream polymerase (n=1), and the next closest(n=2). Analysis of these distances would give information on the PPEP release kinetics, I think... AU - Price, David PY - 2008/04/11/ UR - http://dx.doi.org/10.1016/j.molcel.2008.03.001 ER -