The BRCA2-Interacting Protein BCCIP Functions in RAD51 and BRCA2 Focus Formation and Homologous Recombinational Repair Export

@article{citeulike:2752872, abstract = {Homologous recombinational repair (HRR) of DNA damage is critical for maintaining genome stability and tumor suppression. RAD51 and BRCA2 colocalization in nuclear foci is a hallmark of HRR. BRCA2 has important roles in RAD51 focus formation and HRR of DNA double-strand breaks (DSBs). We previously reported that BCCIP{alpha} interacts with BRCA2. We show that a second isoform, BCCIP{beta}, also interacts with BRCA2 and that this interaction occurs in a region shared by BCCIP{alpha} and BCCIP{beta}. We further show that chromatin-bound BRCA2 colocalizes with BCCIP nuclear foci and that most radiation-induced RAD51 foci colocalize with BCCIP. Reducing BCCIP{alpha} by 90\% or BCCIP{beta} by 50\% by RNA interference markedly reduces RAD51 and BRCA2 foci and reduces HRR of DSBs by 20- to 100-fold. Similarly, reducing BRCA2 by 50\% reduces RAD51 and BCCIP foci. These data indicate that BCCIP is critical for BRCA2- and RAD51-dependent responses to DNA damage and HRR. 10.1128/MCB.25.5.1949-1957.2005}, author = {Lu, Huimei and Guo, Xu and Meng, Xiangbing and Liu, Jingmei and Allen, Chris and Wray, Justin and Nickoloff, Jac A. and Shen, Zhiyuan}, citeulike-article-id = {2752872}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/MCB.25.5.1949-1957.2005}, citeulike-linkout-1 = {http://mcb.asm.org/cgi/content/abstract/25/5/1949}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15713648}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15713648}, day = {1}, doi = {10.1128/MCB.25.5.1949-1957.2005}, journal = {Mol. Cell. Biol.}, keywords = {bccip, brca2, damage, dna, hr, rad51, repair}, month = {March}, number = {5}, pages = {1949--1957}, posted-at = {2008-05-04 06:11:13}, priority = {5}, title = {The BRCA2-Interacting Protein BCCIP Functions in RAD51 and BRCA2 Focus Formation and Homologous Recombinational Repair}, url = {http://dx.doi.org/10.1128/MCB.25.5.1949-1957.2005}, volume = {25}, year = {2005} }

TY - JOUR ID - citeulike:2752872 L3 - citeulike-article-id:2752872 N2 - Homologous recombinational repair (HRR) of DNA damage is critical for maintaining genome stability and tumor suppression. RAD51 and BRCA2 colocalization in nuclear foci is a hallmark of HRR. BRCA2 has important roles in RAD51 focus formation and HRR of DNA double-strand breaks (DSBs). We previously reported that BCCIP{alpha} interacts with BRCA2. We show that a second isoform, BCCIP{beta}, also interacts with BRCA2 and that this interaction occurs in a region shared by BCCIP{alpha} and BCCIP{beta}. We further show that chromatin-bound BRCA2 colocalizes with BCCIP nuclear foci and that most radiation-induced RAD51 foci colocalize with BCCIP. Reducing BCCIP{alpha} by 90% or BCCIP{beta} by 50% by RNA interference markedly reduces RAD51 and BRCA2 foci and reduces HRR of DSBs by 20- to 100-fold. Similarly, reducing BRCA2 by 50% reduces RAD51 and BCCIP foci. These data indicate that BCCIP is critical for BRCA2- and RAD51-dependent responses to DNA damage and HRR. 10.1128/MCB.25.5.1949-1957.2005 IS - 5 JF - Mol. Cell. Biol. EP - 1957 TI - The BRCA2-Interacting Protein BCCIP Functions in RAD51 and BRCA2 Focus Formation and Homologous Recombinational Repair VL - 25 SP - 1949 KW - bccip KW - brca2 KW - damage KW - dna KW - hr KW - rad51 KW - repair AU - Lu, Huimei AU - Guo, Xu AU - Meng, Xiangbing AU - Liu, Jingmei AU - Allen, Chris AU - Wray, Justin AU - Nickoloff, Jac AU - Shen, Zhiyuan PY - 2005/03/01/ UR - http://dx.doi.org/10.1128/MCB.25.5.1949-1957.2005 ER -