The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression — Genes & Development Export

@article{citeulike:3906855, abstract = {Histone monoubiquitylation is implicated in critical regulatory processes. We explored the roles of histone H2B ubiquitylation in human cells by reducing the expression of hBRE1/RNF20, the major H2B-specific E3 ubiquitin ligase. While H2B ubiquitylation is broadly associated with transcribed genes, only a subset of genes was transcriptionally affected by RNF20 depletion and abrogation of H2B ubiquitylation. Gene expression dependent on RNF20 includes histones H2A and H2B and the p53 tumor suppressor. In contrast, RNF20 suppresses the expression of several proto-oncogenes, which reside preferentially in closed chromatin and are modestly transcribed despite bearing marks usually associated with high transcription rates. Remarkably, RNF20 depletion augmented the transcriptional effects of epidermal growth factor (EGF), increased cell migration, and elicited transformation and tumorigenesis. Furthermore, frequent promoter hypermethylation was observed in tumors. RNF20 may thus be a putative tumor suppressor, acting through selective regulation of a distinct subset of genes.}, author = {Shema, Efrat and Tirosh, Itay and Aylon, Yael and Huang, Jing and Ye, Chaoyang and Moskovits, Neta and Raver-Shapira, Nina and Minsky, Neri and Pirngruber, Judith and Tarcic, Gabi and Hublarova, Pavla and Moyal, Lilach and Gana-Weisz, Mali and Shiloh, Yosef and Yarden, Yossef and Johnsen, Steven A. and Vojtesek, Borivoj and Berger, Shelley L. and Oren, Moshe and Pirngruber, J. and Tarcic, G. and Hublarova, P. and Moyal, L. and Gana-Weisz, M. and Shiloh, Y. and Yarden, Y. and Johnsen, S. A. and Vojtesek, B. and Berger, S. L. and Oren, M.}, citeulike-article-id = {3906855}, citeulike-linkout-0 = {http://genesdev.cshlp.org.libproxy.unm.edu/content/22/19/2664}, comment = {This is the paper described in the Espinosa commentary about role of human Bre1 as a tumor suppressor. No time to read the actual article now, but Espinosa review is great. }, journal = {Genes \& Development}, keywords = {bre1, cancer, chip, rnf20, transcription}, pages = {2664--2676}, posted-at = {2009-01-19 06:11:35}, priority = {2}, title = {The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression — Genes \& Development}, url = {http://genesdev.cshlp.org.libproxy.unm.edu/content/22/19/2664}, volume = {22}, year = {2008} }

TY - JOUR ID - citeulike:3906855 L3 - citeulike-article-id:3906855 N2 - Histone monoubiquitylation is implicated in critical regulatory processes. We explored the roles of histone H2B ubiquitylation in human cells by reducing the expression of hBRE1/RNF20, the major H2B-specific E3 ubiquitin ligase. While H2B ubiquitylation is broadly associated with transcribed genes, only a subset of genes was transcriptionally affected by RNF20 depletion and abrogation of H2B ubiquitylation. Gene expression dependent on RNF20 includes histones H2A and H2B and the p53 tumor suppressor. In contrast, RNF20 suppresses the expression of several proto-oncogenes, which reside preferentially in closed chromatin and are modestly transcribed despite bearing marks usually associated with high transcription rates. Remarkably, RNF20 depletion augmented the transcriptional effects of epidermal growth factor (EGF), increased cell migration, and elicited transformation and tumorigenesis. Furthermore, frequent promoter hypermethylation was observed in tumors. RNF20 may thus be a putative tumor suppressor, acting through selective regulation of a distinct subset of genes. JF - Genes & Development EP - 2676 TI - The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression — Genes & Development VL - 22 SP - 2664 KW - bre1 KW - cancer KW - chip KW - rnf20 KW - transcription N1 - This is the paper described in the Espinosa commentary about role of human Bre1 as a tumor suppressor. No time to read the actual article now, but Espinosa review is great. AU - Shema, Efrat AU - Tirosh, Itay AU - Aylon, Yael AU - Huang, Jing AU - Ye, Chaoyang AU - Moskovits, Neta AU - Raver-Shapira, Nina AU - Minsky, Neri AU - Pirngruber, Judith AU - Tarcic, Gabi AU - Hublarova, Pavla AU - Moyal, Lilach AU - Gana-Weisz, Mali AU - Shiloh, Yosef AU - Yarden, Yossef AU - Johnsen, Steven AU - Vojtesek, Borivoj AU - Berger, Shelley AU - Oren, Moshe AU - Pirngruber, J AU - Tarcic, G AU - Hublarova, P AU - Moyal, L AU - Gana-Weisz, M AU - Shiloh, Y AU - Yarden, Y AU - Johnsen, SA AU - Vojtesek, B AU - Berger, SL AU - Oren, M PY - 2008/// UR - http://genesdev.cshlp.org.libproxy.unm.edu/content/22/19/2664 ER -