On-site manipulation of single whole-genome DNA molecules using optical tweezers

by: Hidehiro Oana, Koji Kubo, Kenichi Yoshikawa, Haruyuki Atomi, Tadayuki Imanaka

Applied Physics Letters, Vol. 85, No. 21. (2004), pp. 5090-5092.

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Abstract

In this letter, we describe a noninvasive methodology for manipulating single Mb-size whole-genome DNA molecules. Cells were subjected to osmotic shock and the genome DNA released from the burst cells was transferred to a region of higher salt concentration using optical tweezers. The transferred genome DNA exhibits a conformational transition from a compact state into an elongated state, accompanied by the change in its environment. The applicability of optical tweezers to the on-site manipulation of giant genome DNA is suggested, i.e., lab-on-a-plate. ©2004 American Institute of Physics

@article{citeulike:846277, abstract = {In this letter, we describe a noninvasive methodology for manipulating single Mb-size whole-genome DNA molecules. Cells were subjected to osmotic shock and the genome DNA released from the burst cells was transferred to a region of higher salt concentration using optical tweezers. The transferred genome DNA exhibits a conformational transition from a compact state into an elongated state, accompanied by the change in its environment. The applicability of optical tweezers to the on-site manipulation of giant genome DNA is suggested, i.e., lab-on-a-plate. \©2004 American Institute of Physics}, author = {Oana, Hidehiro and Kubo, Koji and Yoshikawa, Kenichi and Atomi, Haruyuki and Imanaka, Tadayuki }, citeulike-article-id = {846277}, doi = {10.1063/1.1825064}, journal = {Applied Physics Letters}, keywords = {chromatin, optical, single-molecule, tweezers}, number = {21}, pages = {5090--5092}, posted-at = {2008-05-15 05:22:37}, priority = {5}, publisher = {AIP}, title = {On-site manipulation of single whole-genome DNA molecules using optical tweezers}, url = {http://scitation.aip.org/getabs/servlet/GetabsServlet?prog=normal\&id=APPLAB000085000021005090000001\&idtype=cvips\&gifs=yes}, volume = {85}, year = {2004} }

RIS record

TY - JOUR ID - citeulike:846277 L3 - citeulike-article-id:846277 TI - On-site manipulation of single whole-genome DNA molecules using optical tweezers JF - Applied Physics Letters VL - 85 IS - 21 SP - 5090 EP - 5092 PB - AIP N2 - In this letter, we describe a noninvasive methodology for manipulating single Mb-size whole-genome DNA molecules. Cells were subjected to osmotic shock and the genome DNA released from the burst cells was transferred to a region of higher salt concentration using optical tweezers. The transferred genome DNA exhibits a conformational transition from a compact state into an elongated state, accompanied by the change in its environment. The applicability of optical tweezers to the on-site manipulation of giant genome DNA is suggested, i.e., lab-on-a-plate. ©2004 American Institute of Physics KW - chromatin KW - optical KW - single-molecule KW - tweezers AU - Oana, Hidehiro AU - Kubo, Koji AU - Yoshikawa, Kenichi AU - Atomi, Haruyuki AU - Imanaka, Tadayuki PY - 2004/// UR - http://scitation.aip.org/getabs/servlet/GetabsServlet?prog=normal&id=APPLAB000085000021005090000001&idtype=cvips&gifs=yes ER -

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