Electromechanical coupling in the membranes of Shaker-transfected HEK cells Export

@article{citeulike:4384305, abstract = {10.1073/pnas.0808045106 Membranes flex with changes in transmembrane potential as a result of changes in interfacial tension, the Lippman effect. We studied the membrane electromotility of K-transfected HEK-293 cells in real time by using combined patch-clamp atomic force microscopy. In the voltage range where the channels were closed, expression had little effect on electromotility relative to wild-type cells. Depolarization between −120 and −40 mV resulted in a linear upward cantilever deflection equivalent to an increase in membrane tension. However, when depolarized sufficiently for channel opening, the electromotility saturated and only recovered over 10 s of milliseconds. This remarkable loss of motility was associated with channel opening, not ionic flux or movement of the voltage sensors. The IL mutant of , in which the voltage dependence of channel opening but not sensor movement is shifted to more positive potentials, caused the loss of electromotility saturation also to shift to more positive potentials. The temporary loss of electromotility associated with channel opening is probably caused by local buckling of the bilayer as the inner half of the channel expands as expected from X-ray structural data.}, author = {Beyder, Arthur and Sachs, Frederick}, citeulike-article-id = {4384305}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0808045106}, citeulike-linkout-1 = {http://www.pnas.org/content/106/16/6626.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/106/16/6626.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19366664}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19366664}, day = {21}, doi = {10.1073/pnas.0808045106}, journal = {Proceedings of the National Academy of Sciences}, keywords = {biomembranes, flexoelectric, ion\_channels}, month = {April}, number = {16}, pages = {6626--6631}, posted-at = {2009-04-23 12:31:10}, priority = {2}, title = {Electromechanical coupling in the membranes of Shaker-transfected HEK cells}, url = {http://dx.doi.org/10.1073/pnas.0808045106}, volume = {106}, year = {2009} }

TY - JOUR ID - citeulike:4384305 L3 - citeulike-article-id:4384305 N2 - 10.1073/pnas.0808045106 Membranes flex with changes in transmembrane potential as a result of changes in interfacial tension, the Lippman effect. We studied the membrane electromotility of K-transfected HEK-293 cells in real time by using combined patch-clamp atomic force microscopy. In the voltage range where the channels were closed, expression had little effect on electromotility relative to wild-type cells. Depolarization between −120 and −40 mV resulted in a linear upward cantilever deflection equivalent to an increase in membrane tension. However, when depolarized sufficiently for channel opening, the electromotility saturated and only recovered over 10 s of milliseconds. This remarkable loss of motility was associated with channel opening, not ionic flux or movement of the voltage sensors. The IL mutant of , in which the voltage dependence of channel opening but not sensor movement is shifted to more positive potentials, caused the loss of electromotility saturation also to shift to more positive potentials. The temporary loss of electromotility associated with channel opening is probably caused by local buckling of the bilayer as the inner half of the channel expands as expected from X-ray structural data. IS - 16 JF - Proceedings of the National Academy of Sciences EP - 6631 TI - Electromechanical coupling in the membranes of Shaker-transfected HEK cells VL - 106 SP - 6626 KW - biomembranes KW - flexoelectric KW - ion_channels AU - Beyder, Arthur AU - Sachs, Frederick PY - 2009/04/21/ UR - http://dx.doi.org/10.1073/pnas.0808045106 ER -