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Engineering A Uranyl-Specific Binding Protein from NikR13 |
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AbstractA new pick-up line: The first uranyl-selective DNA-binding protein is designed using the E. coli nickel(II)-responsive protein NikR as the template. The resulting NikRprime protein binds uranyl (see picture) with a dissociation constant Kd=53 nM and selectively binds to DNA in the presence of uranyl.
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