Asymmetric nature of lateral pressure profiles in supported lipid membranes and its implications for membrane protein functions Export

@article{citeulike:4785828, abstract = {The study of lipid membrane structure and phase behavior at the nano-scale is of utmost importance due to implications in understanding the interplay of lipids and membrane proteins in biochemical membrane processes. Supported lipid bilayers play a key role in providing the means to understand real biological systems. Yet, with regard to membrane protein activation and their functions, the limitations of supported membranes and the possible artifacts they may induce are weakly understood. Here, we study DPPC (dipalmitoylphosphatidylcholine) bilayers on a weakly hydrophilic substrate using coarse grained simulations and show that the lateral pressure profile of a supported bilayer is distinctly different from the corresponding pressure profile for a free-standing DPPC bilayer. The results indicate that due to the substrate, the lateral pressure profile becomes asymmetric, expressing major peaks at the proximal leaflet of the membrane, implying the membrane to be under strong tension. The results provide a new mechanism to explain malfunctions of transmembrane proteins used in supported bilayers.}, author = {Xing, Chenyue and Ollila, Samuli O. H. and Vattulainen, Ilpo and Faller, Roland}, citeulike-article-id = {4785828}, citeulike-linkout-0 = {http://dx.doi.org/10.1039/b901664c}, citeulike-linkout-1 = {http://www.rsc.org/Publishing/Journals/article.asp?doi=b901664c}, doi = {10.1039/b901664c}, journal = {Soft Matter}, keywords = {biomembranes, mechanics}, posted-at = {2009-06-09 12:28:24}, priority = {2}, publisher = {The Royal Society of Chemistry}, title = {Asymmetric nature of lateral pressure profiles in supported lipid membranes and its implications for membrane protein functions}, url = {http://dx.doi.org/10.1039/b901664c}, year = {2009} }

TY - JOUR ID - citeulike:4785828 L3 - citeulike-article-id:4785828 N2 - The study of lipid membrane structure and phase behavior at the nano-scale is of utmost importance due to implications in understanding the interplay of lipids and membrane proteins in biochemical membrane processes. Supported lipid bilayers play a key role in providing the means to understand real biological systems. Yet, with regard to membrane protein activation and their functions, the limitations of supported membranes and the possible artifacts they may induce are weakly understood. Here, we study DPPC (dipalmitoylphosphatidylcholine) bilayers on a weakly hydrophilic substrate using coarse grained simulations and show that the lateral pressure profile of a supported bilayer is distinctly different from the corresponding pressure profile for a free-standing DPPC bilayer. The results indicate that due to the substrate, the lateral pressure profile becomes asymmetric, expressing major peaks at the proximal leaflet of the membrane, implying the membrane to be under strong tension. The results provide a new mechanism to explain malfunctions of transmembrane proteins used in supported bilayers. JF - Soft Matter TI - Asymmetric nature of lateral pressure profiles in supported lipid membranes and its implications for membrane protein functions PB - The Royal Society of Chemistry KW - biomembranes KW - mechanics AU - Xing, Chenyue AU - Ollila, Samuli AU - Vattulainen, Ilpo AU - Faller, Roland PY - 2009/// UR - http://dx.doi.org/10.1039/b901664c ER -