Cell Viability and DNA Denaturation Measurements by Two-Photon Fluorescence Excitation in CW Al:GaAs Diode Laser Optical Traps Export

@article{citeulike:1539320, abstract = {Cell viability and DNA denaturation are measured through two-photon fluorescence excitation using a single diode laser beam as the trapping and exciting source simultaneously. Two-photon fluorescence emission spectra are presented for CHO cells and T lymphocytes loaded with various fluorescent probes. This single beam method is demonstrated to be a safe tool to monitor the viability of optically trapped cells, even under intense 809 nm diode laser illumination (\~{}106 W/cm2). The dynamics of cellular necrosis is monitored by adding ethanol to the cell suspension during trapping. Thermal damage to heat-treated samples is assessed by recording shifts in the emission spectra from trapped cells loaded with the nucleic acid probe, acridine orange. \©1999 Society of Photo-Optical Instrumentation Engineers.}, author = {Zhang, Z. X. and Sonek, G. J. and Wei, X. B. and Sun, C. and Berns, M. W. and Tromberg, Bruce J.}, citeulike-article-id = {1539320}, citeulike-linkout-0 = {http://scitation.aip.org/getabs/servlet/GetabsServlet?prog=normal&id=JBOPFO000004000002000256000001&idtype=cvips&gifs=yes}, citeulike-linkout-1 = {http://link.aip.org/link/?JBO/4/256}, citeulike-linkout-2 = {http://dx.doi.org/10.1117/1.429948}, doi = {10.1117/1.429948}, journal = {Journal of Biomedical Optics}, keywords = {algaas, aluminum, arsenide, cell, continous, cw, denature, diode, dna, fluorescence, gallium, laser, optical, tweezers, two-photon, viability, wave}, number = {2}, pages = {256--259}, posted-at = {2007-08-07 08:33:19}, priority = {0}, publisher = {SPIE}, title = {Cell Viability and DNA Denaturation Measurements by Two-Photon Fluorescence Excitation in CW Al:GaAs Diode Laser Optical Traps}, url = {http://dx.doi.org/10.1117/1.429948}, volume = {4}, year = {1999} }

TY - JOUR ID - citeulike:1539320 L3 - citeulike-article-id:1539320 N2 - Cell viability and DNA denaturation are measured through two-photon fluorescence excitation using a single diode laser beam as the trapping and exciting source simultaneously. Two-photon fluorescence emission spectra are presented for CHO cells and T lymphocytes loaded with various fluorescent probes. This single beam method is demonstrated to be a safe tool to monitor the viability of optically trapped cells, even under intense 809 nm diode laser illumination (~106 W/cm2). The dynamics of cellular necrosis is monitored by adding ethanol to the cell suspension during trapping. Thermal damage to heat-treated samples is assessed by recording shifts in the emission spectra from trapped cells loaded with the nucleic acid probe, acridine orange. ©1999 Society of Photo-Optical Instrumentation Engineers. IS - 2 JF - Journal of Biomedical Optics EP - 259 TI - Cell Viability and DNA Denaturation Measurements by Two-Photon Fluorescence Excitation in CW Al:GaAs Diode Laser Optical Traps PB - SPIE VL - 4 SP - 256 KW - algaas KW - aluminum KW - arsenide KW - cell KW - continous KW - cw KW - denature KW - diode KW - dna KW - fluorescence KW - gallium KW - laser KW - optical KW - tweezers KW - two-photon KW - viability KW - wave AU - Zhang, ZX AU - Sonek, GJ AU - Wei, XB AU - Sun, C AU - Berns, MW AU - Tromberg, Bruce PY - 1999/// UR - http://dx.doi.org/10.1117/1.429948 ER -