Optically and Magnetically Doped Organically Modified Silica Nanoparticles as Efficient Magnetically Guided Biomarkers for Two-Photon Imaging of Live Cancer Cells Export

@article{citeulike:3171032, abstract = {Abstract: We report here solution-phase synthesis of multifunctional nanoprobes for imaging live cancer cells. More specifically, quantum dots (QDs) and magnetite (Fe3O4) nanoparticles were coencapsulated within organically modified silica (ORMOSIL) nanoparticles. These optically and magnetically doped ORMOSIL nanoparticles were systematically characterized. These ORMOSIL nanoparticles were used for magnetically guided in vitro delivery to a human cancer cell line. Two-photon imaging was used to confirm the uptake of nanoparticle-doped ORMOSIL nanoparticles into the cancer cells. We also demonstrate that synergistic interaction of both magnetic targeting and transferrin-receptor (TfR)-mediated targeting results in robust in vitro cellular uptake, which is significantly higher than that obtained using these two targeting modes individually. Our cell viability study indicates them to be nontoxic. The results illustrate a promising biocompatible nanoparticle platform where multiple imaging and targeting agents can be coincorporated, with the purpose of efficient two-photon bioimaging and magnetically assisted targeting.}, address = {Institute for Lasers, Photonics and Biophotonics and Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, New York 14260-4200, and Department of Physics, The State University of New York at Buffalo, Buffalo, New York 14260-4200}, author = {Law, Wing-Cheung and Yong, Ken-Tye and Roy, Indrajit and Xu, Gaixia and Ding, Hong and Bergey, Earl J. and Zeng, Hao and Prasad, Paras N.}, citeulike-article-id = {3171032}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/jp712090y}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/jp712090y}, day = {29}, doi = {10.1021/jp712090y}, journal = {J. Phys. Chem. C}, keywords = {cancer, cells, citedby, guided, imaging, magnetic, nanoparticles, optical, ormosil, silica, tag, two-photon}, month = {May}, number = {21}, pages = {7972--7977}, posted-at = {2008-08-29 04:34:54}, priority = {2}, title = {Optically and Magnetically Doped Organically Modified Silica Nanoparticles as Efficient Magnetically Guided Biomarkers for Two-Photon Imaging of Live Cancer Cells}, url = {http://dx.doi.org/10.1021/jp712090y}, volume = {112}, year = {2008} }

TY - JOUR ID - citeulike:3171032 L3 - citeulike-article-id:3171032 N2 - Abstract: We report here solution-phase synthesis of multifunctional nanoprobes for imaging live cancer cells. More specifically, quantum dots (QDs) and magnetite (Fe3O4) nanoparticles were coencapsulated within organically modified silica (ORMOSIL) nanoparticles. These optically and magnetically doped ORMOSIL nanoparticles were systematically characterized. These ORMOSIL nanoparticles were used for magnetically guided in vitro delivery to a human cancer cell line. Two-photon imaging was used to confirm the uptake of nanoparticle-doped ORMOSIL nanoparticles into the cancer cells. We also demonstrate that synergistic interaction of both magnetic targeting and transferrin-receptor (TfR)-mediated targeting results in robust in vitro cellular uptake, which is significantly higher than that obtained using these two targeting modes individually. Our cell viability study indicates them to be nontoxic. The results illustrate a promising biocompatible nanoparticle platform where multiple imaging and targeting agents can be coincorporated, with the purpose of efficient two-photon bioimaging and magnetically assisted targeting. IS - 21 JF - J. Phys. Chem. C AD - Institute for Lasers, Photonics and Biophotonics and Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, New York 14260-4200, and Department of Physics, The State University of New York at Buffalo, Buffalo, New York 14260-4200 EP - 7977 TI - Optically and Magnetically Doped Organically Modified Silica Nanoparticles as Efficient Magnetically Guided Biomarkers for Two-Photon Imaging of Live Cancer Cells VL - 112 SP - 7972 KW - cancer KW - cells KW - citedby KW - guided KW - imaging KW - magnetic KW - nanoparticles KW - optical KW - ormosil KW - silica KW - tag KW - two-photon AU - Law, Wing-Cheung AU - Yong, Ken-Tye AU - Roy, Indrajit AU - Xu, Gaixia AU - Ding, Hong AU - Bergey, Earl AU - Zeng, Hao AU - Prasad, Paras PY - 2008/05/29/ UR - http://dx.doi.org/10.1021/jp712090y ER -