The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms Export

@article{citeulike:3718468, abstract = {Abstract\ \  Currently available microbiological techniques are not designed to deal with very slowly growing microorganisms. The enrichment and study of such organisms demands a novel experimental approach. In the present investigation, the sequencing batch reactor (SBR) was applied and optimized for the enrichment and quantitative study of a very slowly growing microbial community which oxidizes ammonium anaerobically. The SBR was shown to be a powerful experimental set-up with the following strong points: (1) efficient biomass retention, (2) a homogeneous distribution of substrates, products and biomass aggregates over the reactor, (3) reliable operation for more than 1 year, and (4) stable conditions under substrate-limiting conditions. Together, these points made possible for the first time the determination of several important physiological parameters such as the biomass yield (0.066  0.01 C-mol/mol ammonium), the maximum specific ammonium consumption rate (45  5 nmol/mg protein/min) and the maximum specific growth rate (0.0027  h−1, doubling time 11 days). In addition, the persisting stable and strongly selective conditions of the SBR led to a high degree of enrichment (74\% of the desired microorganism). This study has demonstrated that the SBR is a powerful tool compared to other techniques used in the past. We suggest that the SBR could be used for the enrichment and quantitative study of a large number of slowly growing microorganisms that are currently out of reach for microbiological research.}, author = {Strous, M. and Heijnen, J. J. and Kuenen, J. G. and Jetten, M. S. M.}, citeulike-article-id = {3718468}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s002530051340}, citeulike-linkout-1 = {http://www.springerlink.com/content/lxveu7fbw84eb0gx}, doi = {10.1007/s002530051340}, journal = {Applied Microbiology and Biotechnology}, keywords = {anammox, methods}, month = {November}, number = {5}, pages = {589--596}, posted-at = {2008-11-27 20:08:36}, priority = {2}, title = {The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms}, url = {http://dx.doi.org/10.1007/s002530051340}, volume = {50}, year = {1998} }

TY - JOUR ID - citeulike:3718468 L3 - citeulike-article-id:3718468 N2 - Abstract   Currently available microbiological techniques are not designed to deal with very slowly growing microorganisms. The enrichment and study of such organisms demands a novel experimental approach. In the present investigation, the sequencing batch reactor (SBR) was applied and optimized for the enrichment and quantitative study of a very slowly growing microbial community which oxidizes ammonium anaerobically. The SBR was shown to be a powerful experimental set-up with the following strong points: (1) efficient biomass retention, (2) a homogeneous distribution of substrates, products and biomass aggregates over the reactor, (3) reliable operation for more than 1 year, and (4) stable conditions under substrate-limiting conditions. Together, these points made possible for the first time the determination of several important physiological parameters such as the biomass yield (0.066  0.01 C-mol/mol ammonium), the maximum specific ammonium consumption rate (45  5 nmol/mg protein/min) and the maximum specific growth rate (0.0027  h−1, doubling time 11 days). In addition, the persisting stable and strongly selective conditions of the SBR led to a high degree of enrichment (74% of the desired microorganism). This study has demonstrated that the SBR is a powerful tool compared to other techniques used in the past. We suggest that the SBR could be used for the enrichment and quantitative study of a large number of slowly growing microorganisms that are currently out of reach for microbiological research. IS - 5 JF - Applied Microbiology and Biotechnology EP - 596 TI - The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms VL - 50 SP - 589 KW - anammox KW - methods AU - Strous, M AU - Heijnen, JJ AU - Kuenen, JG AU - Jetten, MSM PY - 1998/11/16/ UR - http://dx.doi.org/10.1007/s002530051340 ER -