Cell division ring, a new cell division protein and vertical inheritance of a bacterial organelle in anammox planctomycetes. Export

@article{citeulike:5739190, abstract = {Summary Anammox bacteria are members of the phylum Planctomycetes that oxidize ammonium anaerobically and produce a significant part of the atmosphere's dinitrogen gas. They contain a unique bacterial organelle, the anammoxosome, which is the locus of anammox catabolism. While studying anammox cell and anammoxosome division with transmission electron microscopy including electron tomography, we observed a cell division ring in the outermost compartment of dividing anammox cells. In most Bacteria, GTP hydrolysis drives the tubulin-analogue FtsZ to assemble into a ring-like structure at the cell division site where it functions as a scaffold for the molecular machinery that performs cell division. However, the genome of the anammox bacterium 'Candidatus Kuenenia stuttgartiensis' does not encode ftsZ. Genomic analysis of open reading frames with potential GTPase activity indicated a possible novel cell division ring gene: kustd1438, which was unrelated to ftsZ. Immunogold localization specifically localized kustd1438 to the cell division ring. Genomic analyses of other members of the phyla Planctomycetes and Chlamydiae revealed no putative functional homologues of kustd1438, suggesting that it is specific to anammox bacteria. Electron tomography also revealed that the bacterial organelle was elongated along with the rest of the cell and divided equally among daughter cells during the cell division process.}, address = {Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands.; Cellular Architecture and Dynamics, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.; Department of Microbiology and Parasitology (JAF)Centre for Microscopy and Microanalysis (RIW), University of Queensland, Brisbane, QLD 4072, Australia.; Max Planck Institute for Marine Microbiology, Celciusstrasse 1, 28359 Bremen, Germany.}, author = {van Niftrik, Laura and Geerts, Willie J. and van Donselaar, Elly G. and Humbel, Bruno M. and Webb, Richard I. and Harhangi, Harry R. and Camp, Huub Op den J. and Fuerst, John A. and Verkleij, Arie J. and Jetten, Mike S. and Strous, Marc}, citeulike-article-id = {5739190}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1365-2958.2009.06841.x}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19708922}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19708922}, citeulike-linkout-3 = {http://www3.interscience.wiley.com/cgi-bin/abstract/122574982/ABSTRACT}, day = {24}, doi = {10.1111/j.1365-2958.2009.06841.x}, issn = {1365-2958}, journal = {Molecular microbiology}, keywords = {anammox, cell\_biology, electron\_microscopy}, month = {August}, number = {9999}, posted-at = {2009-09-08 10:43:58}, priority = {0}, title = {Cell division ring, a new cell division protein and vertical inheritance of a bacterial organelle in anammox planctomycetes.}, url = {http://dx.doi.org/10.1111/j.1365-2958.2009.06841.x}, volume = {9999}, year = {2009} }

TY - JOUR ID - citeulike:5739190 L3 - citeulike-article-id:5739190 N2 - Summary Anammox bacteria are members of the phylum Planctomycetes that oxidize ammonium anaerobically and produce a significant part of the atmosphere's dinitrogen gas. They contain a unique bacterial organelle, the anammoxosome, which is the locus of anammox catabolism. While studying anammox cell and anammoxosome division with transmission electron microscopy including electron tomography, we observed a cell division ring in the outermost compartment of dividing anammox cells. In most Bacteria, GTP hydrolysis drives the tubulin-analogue FtsZ to assemble into a ring-like structure at the cell division site where it functions as a scaffold for the molecular machinery that performs cell division. However, the genome of the anammox bacterium 'Candidatus Kuenenia stuttgartiensis' does not encode ftsZ. Genomic analysis of open reading frames with potential GTPase activity indicated a possible novel cell division ring gene: kustd1438, which was unrelated to ftsZ. Immunogold localization specifically localized kustd1438 to the cell division ring. Genomic analyses of other members of the phyla Planctomycetes and Chlamydiae revealed no putative functional homologues of kustd1438, suggesting that it is specific to anammox bacteria. Electron tomography also revealed that the bacterial organelle was elongated along with the rest of the cell and divided equally among daughter cells during the cell division process. IS - 9999 JF - Molecular microbiology SN - 1365-2958 AD - Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands.; Cellular Architecture and Dynamics, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.; Department of Microbiology and Parasitology (JAF)Centre for Microscopy and Microanalysis (RIW), University of Queensland, Brisbane, QLD 4072, Australia.; Max Planck Institute for Marine Microbiology, Celciusstrasse 1, 28359 Bremen, Germany. TI - Cell division ring, a new cell division protein and vertical inheritance of a bacterial organelle in anammox planctomycetes. VL - 9999 KW - anammox KW - cell_biology KW - electron_microscopy AU - van Niftrik, Laura AU - Geerts, Willie AU - van Donselaar, Elly AU - Humbel, Bruno AU - Webb, Richard AU - Harhangi, Harry AU - Camp, Huub Op den AU - Fuerst, John AU - Verkleij, Arie AU - Jetten, Mike AU - Strous, Marc PY - 2009/08/24/ UR - http://dx.doi.org/10.1111/j.1365-2958.2009.06841.x ER -