Co-regulated transcriptional networks contribute to natural genetic variation in Drosophila sleep. Export

@article{citeulike:4097904, abstract = {Sleep disorders are common in humans, and sleep loss increases the risk of obesity and diabetes. Studies in Drosophila have revealed molecular pathways and neural tissues regulating sleep; however, genes that maintain genetic variation for sleep in natural populations are unknown. Here, we characterized sleep in 40 wild-derived Drosophila lines and observed abundant genetic variation in sleep architecture. We associated sleep with genome-wide variation in gene expression to identify candidate genes. We independently confirmed that molecular polymorphisms in Catsup (Catecholamines up) are associated with variation in sleep and that P-element mutations in four candidate genes affect sleep and gene expression. Transcripts associated with sleep grouped into biologically plausible genetically correlated transcriptional modules. We confirmed co-regulated gene expression using P-element mutants. Quantitative genetic analysis of natural phenotypic variation is an efficient method for revealing candidate genes and pathways.}, author = {Harbison, Susan T. and Carbone, Mary Anna A. and Ayroles, Julien F. and Stone, Eric A. and Lyman, Richard F. and Mackay, Trudy F.}, citeulike-article-id = {4097904}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ng.330}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/ng.330}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19234472}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19234472}, day = {22}, doi = {10.1038/ng.330}, issn = {1546-1718}, journal = {Nature genetics}, keywords = {co-expression, complex\_traits, drosophila, gene\_regulation, microarray, natural\_variation, network, p-element, sleep}, month = {March}, number = {3}, pages = {371--375}, posted-at = {2009-10-28 09:56:51}, priority = {2}, publisher = {Nature Publishing Group}, title = {Co-regulated transcriptional networks contribute to natural genetic variation in Drosophila sleep.}, url = {http://dx.doi.org/10.1038/ng.330}, volume = {41}, year = {2009} }

TY - JOUR ID - citeulike:4097904 L3 - citeulike-article-id:4097904 N2 - Sleep disorders are common in humans, and sleep loss increases the risk of obesity and diabetes. Studies in Drosophila have revealed molecular pathways and neural tissues regulating sleep; however, genes that maintain genetic variation for sleep in natural populations are unknown. Here, we characterized sleep in 40 wild-derived Drosophila lines and observed abundant genetic variation in sleep architecture. We associated sleep with genome-wide variation in gene expression to identify candidate genes. We independently confirmed that molecular polymorphisms in Catsup (Catecholamines up) are associated with variation in sleep and that P-element mutations in four candidate genes affect sleep and gene expression. Transcripts associated with sleep grouped into biologically plausible genetically correlated transcriptional modules. We confirmed co-regulated gene expression using P-element mutants. Quantitative genetic analysis of natural phenotypic variation is an efficient method for revealing candidate genes and pathways. IS - 3 JF - Nature genetics SN - 1546-1718 EP - 375 TI - Co-regulated transcriptional networks contribute to natural genetic variation in Drosophila sleep. PB - Nature Publishing Group VL - 41 SP - 371 KW - co-expression KW - complex_traits KW - drosophila KW - gene_regulation KW - microarray KW - natural_variation KW - network KW - p-element KW - sleep AU - Harbison, Susan AU - Carbone, Mary Anna AU - Ayroles, Julien AU - Stone, Eric AU - Lyman, Richard AU - Mackay, Trudy PY - 2009/03/22/ UR - http://dx.doi.org/10.1038/ng.330 ER -