Transcriptional regulation of the Drosophila catalase gene by the DRE/DREF system. Export

@article{citeulike:4214512, abstract = {Reactive oxygen species (ROS) cause oxidative stress and aging. The catalase gene is a key component of the cellular antioxidant defense network. However, the molecular mechanisms that regulate catalase gene expression are poorly understood. In this study, we have identified a DNA replication-related element (DRE; 5'-TATCGATA) in the 5'-flanking region of the Drosophila catalase gene. Gel mobility shift assays revealed that a previously identified factor called DREF (DRE- binding factor) binds to the DRE sequence in the Drosophila catalase gene. We used site-directed mutagenesis and in vitro transient transfection assays to establish that expression of the catalase gene is regulated by DREF through the DRE site. To explore the role of DRE/DREF in vivo, we established transgenic flies carrying a catalase-lacZ fusion gene with or without mutation in the DRE. The beta-galactosidase expression patterns of these reporter transgenic lines demonstrated that the catalase gene is upregulated by DREF through the DRE sequence. In addition, we observed suppression of the ectopic DREF-induced rough eye phenotype by a catalase amorphic Cat(n1) allele, indicating that DREF activity is modulated by the intracellular redox state. These results indicate that the DRE/DREF system is a key regulator of catalase gene expression and provide evidence of cross-talk between the DRE/DREF system and the antioxidant defense system.}, author = {Park, S. Y. and Kim, Y. S. and Yang, D. J. and Yoo, M. A.}, citeulike-article-id = {4214512}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/14982956}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=14982956}, issn = {1362-4962}, journal = {Nucleic acids research}, keywords = {catalase, conserved\_regulatory\_element, curated, emsa, reporter\_gene\_assay}, number = {4}, pages = {1318--1324}, posted-at = {2009-03-24 14:38:18}, priority = {0}, title = {Transcriptional regulation of the Drosophila catalase gene by the DRE/DREF system.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/14982956}, volume = {32}, year = {2004} }

TY - JOUR ID - citeulike:4214512 L3 - citeulike-article-id:4214512 N2 - Reactive oxygen species (ROS) cause oxidative stress and aging. The catalase gene is a key component of the cellular antioxidant defense network. However, the molecular mechanisms that regulate catalase gene expression are poorly understood. In this study, we have identified a DNA replication-related element (DRE; 5'-TATCGATA) in the 5'-flanking region of the Drosophila catalase gene. Gel mobility shift assays revealed that a previously identified factor called DREF (DRE- binding factor) binds to the DRE sequence in the Drosophila catalase gene. We used site-directed mutagenesis and in vitro transient transfection assays to establish that expression of the catalase gene is regulated by DREF through the DRE site. To explore the role of DRE/DREF in vivo, we established transgenic flies carrying a catalase-lacZ fusion gene with or without mutation in the DRE. The beta-galactosidase expression patterns of these reporter transgenic lines demonstrated that the catalase gene is upregulated by DREF through the DRE sequence. In addition, we observed suppression of the ectopic DREF-induced rough eye phenotype by a catalase amorphic Cat(n1) allele, indicating that DREF activity is modulated by the intracellular redox state. These results indicate that the DRE/DREF system is a key regulator of catalase gene expression and provide evidence of cross-talk between the DRE/DREF system and the antioxidant defense system. IS - 4 JF - Nucleic acids research SN - 1362-4962 EP - 1324 TI - Transcriptional regulation of the Drosophila catalase gene by the DRE/DREF system. VL - 32 SP - 1318 KW - catalase KW - conserved_regulatory_element KW - curated KW - emsa KW - reporter_gene_assay AU - Park, SY AU - Kim, YS AU - Yang, DJ AU - Yoo, MA PY - 2004/// UR - http://view.ncbi.nlm.nih.gov/pubmed/14982956 ER -