Transcriptional regulation of the Drosophila caudal homeobox gene by DRE/DREF. Export

@article{citeulike:4219875, abstract = {The caudal-related homeobox transcription factors are required for the normal development and differentiation of intestinal cells. Recent reports indicate that misregulation of homeotic gene expression is associated with gastrointestinal cancer in mammals. However, the molecular mechanisms that regulate expression of the caudal-related homeobox genes are poorly understood. In this study, we have identified a DNA replication-related element (DRE) in the 5' flanking region of the Drosophila caudal gene. Gel-mobility shift analysis reveals that three of the four DRE-related sequences in the caudal 5'-flanking region are recognized by the DRE-binding factor (DREF). Deletion and site-directed mutagenesis of these DRE sites results in a considerable reduction in caudal gene promoter activity. Analyses with transgenic flies carrying a caudal-lacZ fusion gene bearing wild-type or mutant DRE sites indicate that the DRE sites are required for caudal expression in vivo. These findings indicate that DRE/DREF is a key regulator of Drosophila caudal homeobox gene expression and suggest that DREs and DREF contribute to intestinal development by regulating caudal gene expression.}, author = {Choi, Y. J. and Choi, T. Y. and Yamaguchi, M. and Matsukage, A. and Kim, Y. S. and Yoo, M. A.}, citeulike-article-id = {4219875}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/15254275}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=15254275}, issn = {1362-4962}, journal = {Nucleic acids research}, keywords = {cad, caudal, cis-regulatory, conserved\_regulatory\_element, curated, dref, drosophila, luciferase}, number = {12}, pages = {3734--3742}, posted-at = {2009-03-25 15:47:59}, priority = {2}, title = {Transcriptional regulation of the Drosophila caudal homeobox gene by DRE/DREF.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/15254275}, volume = {32}, year = {2004} }

TY - JOUR ID - citeulike:4219875 L3 - citeulike-article-id:4219875 N2 - The caudal-related homeobox transcription factors are required for the normal development and differentiation of intestinal cells. Recent reports indicate that misregulation of homeotic gene expression is associated with gastrointestinal cancer in mammals. However, the molecular mechanisms that regulate expression of the caudal-related homeobox genes are poorly understood. In this study, we have identified a DNA replication-related element (DRE) in the 5' flanking region of the Drosophila caudal gene. Gel-mobility shift analysis reveals that three of the four DRE-related sequences in the caudal 5'-flanking region are recognized by the DRE-binding factor (DREF). Deletion and site-directed mutagenesis of these DRE sites results in a considerable reduction in caudal gene promoter activity. Analyses with transgenic flies carrying a caudal-lacZ fusion gene bearing wild-type or mutant DRE sites indicate that the DRE sites are required for caudal expression in vivo. These findings indicate that DRE/DREF is a key regulator of Drosophila caudal homeobox gene expression and suggest that DREs and DREF contribute to intestinal development by regulating caudal gene expression. IS - 12 JF - Nucleic acids research SN - 1362-4962 EP - 3742 TI - Transcriptional regulation of the Drosophila caudal homeobox gene by DRE/DREF. VL - 32 SP - 3734 KW - cad KW - caudal KW - cis-regulatory KW - conserved_regulatory_element KW - curated KW - dref KW - drosophila KW - luciferase AU - Choi, YJ AU - Choi, TY AU - Yamaguchi, M AU - Matsukage, A AU - Kim, YS AU - Yoo, MA PY - 2004/// UR - http://view.ncbi.nlm.nih.gov/pubmed/15254275 ER -