Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells Export

@article{citeulike:2393298, abstract = {Induced pluripotent stem (iPS) cells have been generated from mouse and human fibroblasts by the retroviral transduction of four transcription factors. However, the cell origins and molecular mechanisms of iPS cell induction remain elusive. This report describes the generation of iPS cells from adult mouse hepatocytes and gastric epithelial cells. These iPS cell clones appear to be equivalent to embryonic stem cells in gene expression and are competent to generate germline chimeras. Genetic lineage tracings show that liver-derived iPS cells are derived from albumin-expressing cells. No common retroviral integration sites are found among multiple clones. These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required. 10.1126/science.1154884}, address = {Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan; Department of Gastroenterology and Hepatology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.}, author = {Aoi, Takashi and Yae, Kojiro and Nakagawa, Masato and Ichisaka, Tomoko and Okita, Keisuke and Takahashi, Kazutoshi and Chiba, Tsutomu and Yamanaka, Shinya}, citeulike-article-id = {2393298}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1154884}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/321/5889/699}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18276851}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18276851}, day = {1}, doi = {10.1126/science.1154884}, issn = {1095-9203}, journal = {Science}, keywords = {stemcells}, month = {August}, number = {5889}, pages = {699--702}, posted-at = {2008-08-01 16:43:52}, priority = {2}, title = {Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells}, url = {http://dx.doi.org/10.1126/science.1154884}, volume = {321}, year = {2008} }

TY - JOUR ID - citeulike:2393298 L3 - citeulike-article-id:2393298 N2 - Induced pluripotent stem (iPS) cells have been generated from mouse and human fibroblasts by the retroviral transduction of four transcription factors. However, the cell origins and molecular mechanisms of iPS cell induction remain elusive. This report describes the generation of iPS cells from adult mouse hepatocytes and gastric epithelial cells. These iPS cell clones appear to be equivalent to embryonic stem cells in gene expression and are competent to generate germline chimeras. Genetic lineage tracings show that liver-derived iPS cells are derived from albumin-expressing cells. No common retroviral integration sites are found among multiple clones. These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required. 10.1126/science.1154884 IS - 5889 JF - Science SN - 1095-9203 AD - Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan; Department of Gastroenterology and Hepatology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan. EP - 702 TI - Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells VL - 321 SP - 699 KW - stemcells AU - Aoi, Takashi AU - Yae, Kojiro AU - Nakagawa, Masato AU - Ichisaka, Tomoko AU - Okita, Keisuke AU - Takahashi, Kazutoshi AU - Chiba, Tsutomu AU - Yamanaka, Shinya PY - 2008/08/01/ UR - http://dx.doi.org/10.1126/science.1154884 ER -