Discovery of insect and human dengue virus host factors Export

@article{citeulike:4383910, abstract = {Dengue fever is the most frequent arthropod-borne viral disease of humans, with almost half of the world\&\#25;s population at risk of infection. The high prevalence, lack of an effective vaccine, and absence of specific treatment conspire to make dengue fever a global public health threat. Given their compact genomes, dengue viruses (DENV-1\&\#19;4) and other flaviviruses probably require an extensive number of host factors; however, only a limited number of human, and an even smaller number of insect host factors, have been identified. Here we identify insect host factors required for DENV-2 propagation, by carrying out a genome-wide RNA interference screen in Drosophila melanogaster cells using a well-established 22,632 double-stranded RNA library. This screen identified 116 candidate dengue virus host factors (DVHFs). Although some were previously associated with flaviviruses (for example, V-ATPases and \&\#177;-glucosidases), most of the DVHFs were newly implicated in dengue virus propagation. The dipteran DVHFs had 82 readily recognizable human homologues and, using a targeted short-interfering-RNA screen, we showed that 42 of these are human DVHFs. This indicates notable conservation of required factors between dipteran and human hosts. This work suggests new approaches to control infection in the insect vector and the mammalian host.}, author = {Sessions, October M. and Barrows, Nicholas J. and Souza-Neto, Jayme A. and Robinson, Timothy J. and Hershey, Christine L. and Rodgers, Mary A. and Ramirez, Jose L. and Dimopoulos, George and Yang, Priscilla L. and Pearson, James L. and Garcia-Blanco, Mariano A.}, citeulike-article-id = {4383910}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature07967}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature07967}, doi = {10.1038/nature07967}, issn = {0028-0836}, journal = {Nature}, keywords = {flavivirus, replication, rnai}, number = {7241}, pages = {1047--1050}, posted-at = {2009-04-26 16:25:47}, priority = {2}, publisher = {Nature Publishing Group}, title = {Discovery of insect and human dengue virus host factors}, url = {http://dx.doi.org/10.1038/nature07967}, volume = {458} }

TY - JOUR ID - citeulike:4383910 L3 - citeulike-article-id:4383910 N2 - Dengue fever is the most frequent arthropod-borne viral disease of humans, with almost half of the worlds population at risk of infection. The high prevalence, lack of an effective vaccine, and absence of specific treatment conspire to make dengue fever a global public health threat. Given their compact genomes, dengue viruses (DENV-14) and other flaviviruses probably require an extensive number of host factors; however, only a limited number of human, and an even smaller number of insect host factors, have been identified. Here we identify insect host factors required for DENV-2 propagation, by carrying out a genome-wide RNA interference screen in Drosophila melanogaster cells using a well-established 22,632 double-stranded RNA library. This screen identified 116 candidate dengue virus host factors (DVHFs). Although some were previously associated with flaviviruses (for example, V-ATPases and ±-glucosidases), most of the DVHFs were newly implicated in dengue virus propagation. The dipteran DVHFs had 82 readily recognizable human homologues and, using a targeted short-interfering-RNA screen, we showed that 42 of these are human DVHFs. This indicates notable conservation of required factors between dipteran and human hosts. This work suggests new approaches to control infection in the insect vector and the mammalian host. IS - 7241 JF - Nature SN - 0028-0836 EP - 1050 TI - Discovery of insect and human dengue virus host factors PB - Nature Publishing Group VL - 458 SP - 1047 KW - flavivirus KW - replication KW - rnai AU - Sessions, October AU - Barrows, Nicholas AU - Souza-Neto, Jayme AU - Robinson, Timothy AU - Hershey, Christine AU - Rodgers, Mary AU - Ramirez, Jose AU - Dimopoulos, George AU - Yang, Priscilla AU - Pearson, James AU - Garcia-Blanco, Mariano UR - http://dx.doi.org/10.1038/nature07967 ER -