Cholesterol biosynthesis modulation regulates dengue viral replication Export

@article{citeulike:4536197, abstract = {We performed a focused siRNA screen in an A549 dengue type 2 New Guinea C subgenomic replicon cell line (Rluc-replicon) that contains a Renilla luciferase cassette. We found that siRNA mediated knock down of mevalonate diphospho decarboxylase (MVD) inhibited viral replication of the Rluc-replicon and DEN-2 NGC live virus replication in A549 cells. When the Rluc-replicon A459 cells were grown in delipidated media the replicon expression was suppressed and MVD knock down could further sensitize Renilla expression. Hymeglusin and zaragozic acid A could inhibit DEN-2 NGC live virus replication in K562 cells, while lovastatin could inhibit DEN-2 NGC live virus replication in human peripheral blood mononuclear cells. Renilla expression could be rescued in fluvastatin treated A549 Rluc-replicon cells after the addition of mevalonate, and partially restored with geranylgeranyl pyrophosphate, or farnesyl pyrophosphate. Our data suggest genetic and pharmacological modulation of cholesterol biosynthesis can regulate dengue virus replication.}, author = {Rothwell, Christopher and Lebreton, Aude and Young and Lim, Joanne Y. and Liu, Wei and Vasudevan, Subhash and Labow, Mark and Gu, Feng and Gaither, Alex L.}, citeulike-article-id = {4536197}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.virol.2009.03.025}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0042682209002025}, day = {05}, doi = {10.1016/j.virol.2009.03.025}, issn = {00426822}, journal = {Virology}, keywords = {entry, flavivirus, raft}, month = {May}, posted-at = {2009-06-10 11:12:03}, priority = {2}, title = {Cholesterol biosynthesis modulation regulates dengue viral replication}, url = {http://dx.doi.org/10.1016/j.virol.2009.03.025}, year = {2009} }

TY - JOUR ID - citeulike:4536197 L3 - citeulike-article-id:4536197 N2 - We performed a focused siRNA screen in an A549 dengue type 2 New Guinea C subgenomic replicon cell line (Rluc-replicon) that contains a Renilla luciferase cassette. We found that siRNA mediated knock down of mevalonate diphospho decarboxylase (MVD) inhibited viral replication of the Rluc-replicon and DEN-2 NGC live virus replication in A549 cells. When the Rluc-replicon A459 cells were grown in delipidated media the replicon expression was suppressed and MVD knock down could further sensitize Renilla expression. Hymeglusin and zaragozic acid A could inhibit DEN-2 NGC live virus replication in K562 cells, while lovastatin could inhibit DEN-2 NGC live virus replication in human peripheral blood mononuclear cells. Renilla expression could be rescued in fluvastatin treated A549 Rluc-replicon cells after the addition of mevalonate, and partially restored with geranylgeranyl pyrophosphate, or farnesyl pyrophosphate. Our data suggest genetic and pharmacological modulation of cholesterol biosynthesis can regulate dengue virus replication. JF - Virology SN - 00426822 TI - Cholesterol biosynthesis modulation regulates dengue viral replication KW - entry KW - flavivirus KW - raft AU - Rothwell, Christopher AU - Lebreton, Aude AU - Young AU - Lim, Joanne AU - Liu, Wei AU - Vasudevan, Subhash AU - Labow, Mark AU - Gu, Feng AU - Gaither, Alex PY - 2009/05/05/ UR - http://dx.doi.org/10.1016/j.virol.2009.03.025 ER -