RIG-I is cleaved during picornavirus infection. Export

@article{citeulike:5274253, abstract = {The innate immune system senses RNA virus infections through membrane-bound Toll-like receptors or the cytoplasmic proteins RIG-I and MDA-5. RIG-I is believed to recognize the 5'-triphosphate present on many viral RNAs, and hence is important for sensing infections by paramyxoviruses, influenza viruses, rhabdoviruses, and flaviviruses. MDA-5 recognizes dsRNA, and senses infection with picornaviruses, whose RNA 5'-ends are linked to a viral protein, VPg, not a 5'-triphosphate. We previously showed that MDA-5 is degraded in cells infected with different picornaviruses, and suggested that such cleavage might be a mechanism to antagonize production of type I IFN in response to viral infection. Here we examined the state of RIG-I during picornavirus infection. RIG-I is degraded in cells infected with poliovirus, rhinoviruses, echovirus, and encephalomyocarditis virus. In contrast to MDA-5, cleavage of RIG-I is not accomplished by cellular caspases or the proteasome. Rather, the viral proteinase 3C(pro) cleaves RIG-I, both in vitro and in cells. Cleavage of RIG-I during picornavirus infection may constitute another mechanism for attenuating the innate response to viral infection.}, author = {Barral, Paola M. and Sarkar, Devanand and Fisher, Paul B. and Racaniello, Vincent R.}, citeulike-article-id = {5274253}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.virol.2009.06.045}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0042682209004073}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19628239}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19628239}, day = {1}, doi = {10.1016/j.virol.2009.06.045}, issn = {1096-0341}, journal = {Virology}, keywords = {innate, interferon}, month = {September}, number = {2}, pages = {171--176}, posted-at = {2009-08-19 13:48:14}, priority = {2}, title = {RIG-I is cleaved during picornavirus infection.}, url = {http://dx.doi.org/10.1016/j.virol.2009.06.045}, volume = {391}, year = {2009} }

TY - JOUR ID - citeulike:5274253 L3 - citeulike-article-id:5274253 N2 - The innate immune system senses RNA virus infections through membrane-bound Toll-like receptors or the cytoplasmic proteins RIG-I and MDA-5. RIG-I is believed to recognize the 5'-triphosphate present on many viral RNAs, and hence is important for sensing infections by paramyxoviruses, influenza viruses, rhabdoviruses, and flaviviruses. MDA-5 recognizes dsRNA, and senses infection with picornaviruses, whose RNA 5'-ends are linked to a viral protein, VPg, not a 5'-triphosphate. We previously showed that MDA-5 is degraded in cells infected with different picornaviruses, and suggested that such cleavage might be a mechanism to antagonize production of type I IFN in response to viral infection. Here we examined the state of RIG-I during picornavirus infection. RIG-I is degraded in cells infected with poliovirus, rhinoviruses, echovirus, and encephalomyocarditis virus. In contrast to MDA-5, cleavage of RIG-I is not accomplished by cellular caspases or the proteasome. Rather, the viral proteinase 3C(pro) cleaves RIG-I, both in vitro and in cells. Cleavage of RIG-I during picornavirus infection may constitute another mechanism for attenuating the innate response to viral infection. IS - 2 JF - Virology SN - 1096-0341 EP - 176 TI - RIG-I is cleaved during picornavirus infection. VL - 391 SP - 171 KW - innate KW - interferon AU - Barral, Paola AU - Sarkar, Devanand AU - Fisher, Paul AU - Racaniello, Vincent PY - 2009/09/01/ UR - http://dx.doi.org/10.1016/j.virol.2009.06.045 ER -