The Infectious Molecular Clone and Pseudotyped Virus Models of Human Immunodeficiency Virus Type 1 Exhibit Significant Differences in Virion Composition with Only Moderate Differences in Infectivity and Inhibition Sensitivity Export

@article{citeulike:5486108, abstract = {Two frequently employed methods for generating well-characterized, genetically defined infectious human immunodeficiency virus type 1 in vitro include the use of infectious molecular clones (IMCs) and pseudoviruses (PVs) competent for single-round infection. We compared six matched pairs of IMCs and PVs. The relative amounts of Env incorporated and efficiency of cleavage differed substantially between the two systems. Altering the ratio of proviral genome and env expression plasmids can produce pseudovirions that are structurally more similar to the matched IMCs. Differences in Env incorporation and cleavage translated into moderate differences in assays infectivity and sensitivity to neutralizing antibodies and entry inhibitors. 10.1128/JVI.00423-09}, author = {Provine, Nicholas M. and Puryear, Wendy B. and Wu, Xueling and Overbaugh, Julie and Haigwood, Nancy L.}, citeulike-article-id = {5486108}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JVI.00423-09}, citeulike-linkout-1 = {http://jvi.asm.org/cgi/content/abstract/83/17/9002}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19535443}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19535443}, day = {1}, doi = {10.1128/JVI.00423-09}, journal = {J. Virol.}, keywords = {hiv, neutralization}, month = {September}, number = {17}, pages = {9002--9007}, posted-at = {2009-08-19 12:32:21}, priority = {2}, title = {The Infectious Molecular Clone and Pseudotyped Virus Models of Human Immunodeficiency Virus Type 1 Exhibit Significant Differences in Virion Composition with Only Moderate Differences in Infectivity and Inhibition Sensitivity}, url = {http://dx.doi.org/10.1128/JVI.00423-09}, volume = {83}, year = {2009} }

TY - JOUR ID - citeulike:5486108 L3 - citeulike-article-id:5486108 N2 - Two frequently employed methods for generating well-characterized, genetically defined infectious human immunodeficiency virus type 1 in vitro include the use of infectious molecular clones (IMCs) and pseudoviruses (PVs) competent for single-round infection. We compared six matched pairs of IMCs and PVs. The relative amounts of Env incorporated and efficiency of cleavage differed substantially between the two systems. Altering the ratio of proviral genome and env expression plasmids can produce pseudovirions that are structurally more similar to the matched IMCs. Differences in Env incorporation and cleavage translated into moderate differences in assays infectivity and sensitivity to neutralizing antibodies and entry inhibitors. 10.1128/JVI.00423-09 IS - 17 JF - J. Virol. EP - 9007 TI - The Infectious Molecular Clone and Pseudotyped Virus Models of Human Immunodeficiency Virus Type 1 Exhibit Significant Differences in Virion Composition with Only Moderate Differences in Infectivity and Inhibition Sensitivity VL - 83 SP - 9002 KW - hiv KW - neutralization AU - Provine, Nicholas AU - Puryear, Wendy AU - Wu, Xueling AU - Overbaugh, Julie AU - Haigwood, Nancy PY - 2009/09/01/ UR - http://dx.doi.org/10.1128/JVI.00423-09 ER -