Functional and genetic analysis of coreceptor usage by dualtropic HIV-1 subtype C isolates Export

@article{citeulike:5490230, abstract = {It is widely documented that a complete switch from the predominant CCR5 (R5) to CXCR4 (X4) phenotype is less common for HIV-1 subtype C (HIV-1C) compared to other major subtypes. We investigated whether dualtropic HIV-1C isolates represented dualtropic, mixed R5 and X4 clones or both. Thirty of 35 functional HIV-1 env clones generated by bulk PCR amplification from peripheral blood mononuclear cells (PBMCs) infected with seven dualtropic HIV-1C isolates utilized CXCR4 exclusively. Five of 35 clones displayed dualtropism. Endpoint dilution of one isolate did not yield a substantial proportion of R5-monotropic env clones. Sequence-based predictive algorithms showed that env sequences from PBMCs, CXCR4 or CCR5-expressing cell lines were indistinguishable and all possessed X4/dualtropic characteristics. We describe HIV-1C CXCR4-tropic env sequence features. Our results suggest a dramatic loss of CCR5 monotropism as dualtropism emerges in HIV-1C which has important implications for the use of coreceptor antagonists in therapeutic strategies for this subtype.}, author = {Singh, Ashika and Page, Taryn and Moore, Penny L. and Allgaier, Rachel L. and Hiramen, Keshni and Coovadia, Hoosen M. and Walker, Bruce D. and Morris, Lynn and Ndung'u, Thumbi}, citeulike-article-id = {5490230}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.virol.2009.07.021}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0042682209004474}, day = {10}, doi = {10.1016/j.virol.2009.07.021}, issn = {00426822}, journal = {Virology}, keywords = {entry, evolution, hiv, macrophage, receptor}, month = {October}, number = {1}, pages = {56--67}, posted-at = {2009-10-02 09:05:28}, priority = {2}, title = {Functional and genetic analysis of coreceptor usage by dualtropic HIV-1 subtype C isolates}, url = {http://dx.doi.org/10.1016/j.virol.2009.07.021}, volume = {393}, year = {2009} }

TY - JOUR ID - citeulike:5490230 L3 - citeulike-article-id:5490230 N2 - It is widely documented that a complete switch from the predominant CCR5 (R5) to CXCR4 (X4) phenotype is less common for HIV-1 subtype C (HIV-1C) compared to other major subtypes. We investigated whether dualtropic HIV-1C isolates represented dualtropic, mixed R5 and X4 clones or both. Thirty of 35 functional HIV-1 env clones generated by bulk PCR amplification from peripheral blood mononuclear cells (PBMCs) infected with seven dualtropic HIV-1C isolates utilized CXCR4 exclusively. Five of 35 clones displayed dualtropism. Endpoint dilution of one isolate did not yield a substantial proportion of R5-monotropic env clones. Sequence-based predictive algorithms showed that env sequences from PBMCs, CXCR4 or CCR5-expressing cell lines were indistinguishable and all possessed X4/dualtropic characteristics. We describe HIV-1C CXCR4-tropic env sequence features. Our results suggest a dramatic loss of CCR5 monotropism as dualtropism emerges in HIV-1C which has important implications for the use of coreceptor antagonists in therapeutic strategies for this subtype. IS - 1 JF - Virology SN - 00426822 EP - 67 TI - Functional and genetic analysis of coreceptor usage by dualtropic HIV-1 subtype C isolates VL - 393 SP - 56 KW - entry KW - evolution KW - hiv KW - macrophage KW - receptor AU - Singh, Ashika AU - Page, Taryn AU - Moore, Penny AU - Allgaier, Rachel AU - Hiramen, Keshni AU - Coovadia, Hoosen AU - Walker, Bruce AU - Morris, Lynn AU - Ndung'u, Thumbi PY - 2009/10/10/ UR - http://dx.doi.org/10.1016/j.virol.2009.07.021 ER -