Detection of an Infectious Retrovirus, XMRV, in Blood Cells of Patients with Chronic Fatigue Syndrome Export

@article{citeulike:5915847, abstract = {Chronic fatigue syndrome (CFS) is a debilitating disease of unknown etiology that is estimated to affect 17 million people worldwide. Studying peripheral blood mononuclear cells (PBMCs) from CFS patients, we identified DNA from a human gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV), in 68 of 101 patients (67\%) as compared to 8 of 218 (3.7\%) healthy controls. Cell culture experiments revealed that patient-derived XMRV is infectious and that both cell-associated and cell-free transmission of the virus are possible. Secondary viral infections were established in uninfected primary lymphocytes and indicator cell lines after their exposure to activated PBMCs, B cells, T cells, or plasma derived from CFS patients. These findings raise the possibility that XMRV may be a contributing factor in the pathogenesis of CFS. 10.1126/science.1179052}, author = {Lombardi, Vincent C. and Ruscetti, Francis W. and Das Gupta, Jaydip and Pfost, Max A. and Hagen, Kathryn S. and Peterson, Daniel L. and Ruscetti, Sandra K. and Bagni, Rachel K. and Petrow-Sadowski, Cari and Gold, Bert and Dean, Michael and Silverman, Robert H. and Mikovits, Judy A.}, citeulike-article-id = {5915847}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1179052}, citeulike-linkout-1 = {http://www.sciencemag.org/content/1179052/.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/1179052/.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19815723}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19815723}, day = {23}, doi = {10.1126/science.1179052}, issn = {1095-9203}, journal = {Science}, keywords = {evolution, human, retrovirus}, month = {October}, number = {5952}, pages = {585--589}, posted-at = {2009-10-26 17:22:04}, priority = {2}, title = {Detection of an Infectious Retrovirus, XMRV, in Blood Cells of Patients with Chronic Fatigue Syndrome}, url = {http://dx.doi.org/10.1126/science.1179052}, volume = {326}, year = {2009} }

TY - JOUR ID - citeulike:5915847 L3 - citeulike-article-id:5915847 N2 - Chronic fatigue syndrome (CFS) is a debilitating disease of unknown etiology that is estimated to affect 17 million people worldwide. Studying peripheral blood mononuclear cells (PBMCs) from CFS patients, we identified DNA from a human gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV), in 68 of 101 patients (67%) as compared to 8 of 218 (3.7%) healthy controls. Cell culture experiments revealed that patient-derived XMRV is infectious and that both cell-associated and cell-free transmission of the virus are possible. Secondary viral infections were established in uninfected primary lymphocytes and indicator cell lines after their exposure to activated PBMCs, B cells, T cells, or plasma derived from CFS patients. These findings raise the possibility that XMRV may be a contributing factor in the pathogenesis of CFS. 10.1126/science.1179052 IS - 5952 JF - Science SN - 1095-9203 EP - 589 TI - Detection of an Infectious Retrovirus, XMRV, in Blood Cells of Patients with Chronic Fatigue Syndrome VL - 326 SP - 585 KW - evolution KW - human KW - retrovirus AU - Lombardi, Vincent AU - Ruscetti, Francis AU - Das Gupta, Jaydip AU - Pfost, Max AU - Hagen, Kathryn AU - Peterson, Daniel AU - Ruscetti, Sandra AU - Bagni, Rachel AU - Petrow-Sadowski, Cari AU - Gold, Bert AU - Dean, Michael AU - Silverman, Robert AU - Mikovits, Judy PY - 2009/10/23/ UR - http://dx.doi.org/10.1126/science.1179052 ER -