Human Immunodeficiency Virus Type 1 Envelope gp120-Induced Partial T-Cell Receptor Signaling Creates an F-Actin-Depleted Zone in the Virological Synapse Export

@article{citeulike:5920758, abstract = {Cell-to-cell transmission of human immunodeficiency virus type 1 (HIV-1) occurs via a virological synapse (VS), a tight cell-cell junction formed between HIV-infected cells and target cells in which the HIV-1-infected cell polarizes and releases virions toward the noninfected target cell in a gp120- and intercellular adhesion molecule 1 (ICAM-1)-dependent process. The response of the target cell has been less studied. We utilized supported planar bilayers presenting gp120 and ICAM-1 as a reductionist model for the infected-cell membrane and investigated its effect on the target CD4 T cell. This study shows that HIV-1 gp120 interaction with its receptors is initially organized into microclusters that undergo F-actin-dependent consolidation into a central supramolecular activation complex (cSMAC). Src kinases are active in both gp120 microclusters and in the VS cSMAC. The early T-cell receptor (TCR) signaling machinery is partially activated at the VS, and signaling does not propagate to trigger Ca2+ elevation or increase CD69 expression. However, these partial TCR signals act locally to create an F-actin-depleted zone. We propose a model in which the F-actin-depleted zone formed within the target CD4 T cell enhances the reception of virions by releasing the physical barrier for HIV-1 entry and facilitating postentry events. 10.1128/JVI.01440-09}, author = {Vasiliver-Shamis, Gaia and Cho, Michael W. and Hioe, Catarina E. and Dustin, Michael L.}, citeulike-article-id = {5920758}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JVI.01440-09}, citeulike-linkout-1 = {http://jvi.asm.org/content/83/21/11341.abstract}, citeulike-linkout-2 = {http://jvi.asm.org/content/83/21/11341.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19710135}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19710135}, day = {1}, doi = {10.1128/JVI.01440-09}, journal = {J. Virol.}, keywords = {entry, hiv}, month = {November}, number = {21}, pages = {11341--11355}, posted-at = {2009-10-11 10:04:14}, priority = {2}, title = {Human Immunodeficiency Virus Type 1 Envelope gp120-Induced Partial T-Cell Receptor Signaling Creates an F-Actin-Depleted Zone in the Virological Synapse}, url = {http://dx.doi.org/10.1128/JVI.01440-09}, volume = {83}, year = {2009} }

TY - JOUR ID - citeulike:5920758 L3 - citeulike-article-id:5920758 N2 - Cell-to-cell transmission of human immunodeficiency virus type 1 (HIV-1) occurs via a virological synapse (VS), a tight cell-cell junction formed between HIV-infected cells and target cells in which the HIV-1-infected cell polarizes and releases virions toward the noninfected target cell in a gp120- and intercellular adhesion molecule 1 (ICAM-1)-dependent process. The response of the target cell has been less studied. We utilized supported planar bilayers presenting gp120 and ICAM-1 as a reductionist model for the infected-cell membrane and investigated its effect on the target CD4 T cell. This study shows that HIV-1 gp120 interaction with its receptors is initially organized into microclusters that undergo F-actin-dependent consolidation into a central supramolecular activation complex (cSMAC). Src kinases are active in both gp120 microclusters and in the VS cSMAC. The early T-cell receptor (TCR) signaling machinery is partially activated at the VS, and signaling does not propagate to trigger Ca2+ elevation or increase CD69 expression. However, these partial TCR signals act locally to create an F-actin-depleted zone. We propose a model in which the F-actin-depleted zone formed within the target CD4 T cell enhances the reception of virions by releasing the physical barrier for HIV-1 entry and facilitating postentry events. 10.1128/JVI.01440-09 IS - 21 JF - J. Virol. EP - 11355 TI - Human Immunodeficiency Virus Type 1 Envelope gp120-Induced Partial T-Cell Receptor Signaling Creates an F-Actin-Depleted Zone in the Virological Synapse VL - 83 SP - 11341 KW - entry KW - hiv AU - Vasiliver-Shamis, Gaia AU - Cho, Michael AU - Hioe, Catarina AU - Dustin, Michael PY - 2009/11/01/ UR - http://dx.doi.org/10.1128/JVI.01440-09 ER -