Slipped-strand mispairing in a plastid gene: rpoC2 in grasses (Poaceae). Export

@article{Cummings1994, abstract = {An exception to the generally conservative nature of plastid gene evolution is the gene coding for the beta" subunit of RNA polymerase, rpoC2. Previous work by others has shown that maize and rice have an insertion in the coding region of rpoC2, relative to spinach and tobacco. To assess the distribution of this extra coding sequence, we surveyed a broad phylogenetic sample comprising 55 species from 17 angiosperm families by using Southern hybridization. The extra coding sequence is restricted to the grasses (Poaceae). DNA sequence analysis of 11 species from all five subfamilies within the grass family demonstrates that the extra sequence in the coding region of rpoC2 is a repetitive array that exhibits more than a twofold increase in nucleotide substitution, as well as a large number of insertion/deletion events, relative to the adjacent flanking sequences. The structure of the array suggests that slipped-strand mispairing causes the repeated motifs and adds to the mechanisms through which the coding sequence of plastid genes are known to evolve. Phylogenetic analyses based on the sequence data from grass species support several relationships previously suggested by morphological work, but they are ambiguous about broad relationships within the family.}, author = {Cummings, M. P. and King, L. M. and Kellogg, E. A.}, citeulike-article-id = {757431}, journal = {Mol. Biol. Evol.}, keywords = {5s, 8121278, acid, aerobiosis, alcohol, alignment, amino, anaerobiosis, angiosperms, animals, arabidopsis, base, bibtex-import, c, cell, cereals, cerevisiae, chloroplasts, comparative, complex, conformation, cytochrome, data, dehydrogenase, diploidy, dna, dna-directed, electron, enzyme, evolution, expression, family, fungal, fungi, gene, genes, genetic, genetics, govt, group, haplotypes, homeobox, homeodomain, homology, humans, immunoglobulin, induction, iv, kinetics, mays, models, molecular, multigene, mutation, non-phs, non-us, nucleic, nucleus, oxygen, phs, phylogeny, plant, plants, plastids, poaceae, polymerases, polymorphism, primers, protein, proteins, region, regulation, repetitive, research, ribosomal, rna, saccharomyces, secondary, selection, sequence, sequences, species, specificity, structural, structure, study, support, transcription, transport, triticum, us, variable, variation, zea}, month = {Jan}, number = {1}, pages = {1--8}, pdf = {Cummings1994.pdf}, posted-at = {2006-07-13 16:23:22}, priority = {2}, title = {Slipped-strand mispairing in a plastid gene: rpo{C}2 in grasses ({P}oaceae).}, volume = {11}, year = {1994} }

TY - JOUR ID - Cummings1994 L3 - citeulike-article-id:757431 N2 - An exception to the generally conservative nature of plastid gene evolution is the gene coding for the beta" subunit of RNA polymerase, rpoC2. Previous work by others has shown that maize and rice have an insertion in the coding region of rpoC2, relative to spinach and tobacco. To assess the distribution of this extra coding sequence, we surveyed a broad phylogenetic sample comprising 55 species from 17 angiosperm families by using Southern hybridization. The extra coding sequence is restricted to the grasses (Poaceae). DNA sequence analysis of 11 species from all five subfamilies within the grass family demonstrates that the extra sequence in the coding region of rpoC2 is a repetitive array that exhibits more than a twofold increase in nucleotide substitution, as well as a large number of insertion/deletion events, relative to the adjacent flanking sequences. The structure of the array suggests that slipped-strand mispairing causes the repeated motifs and adds to the mechanisms through which the coding sequence of plastid genes are known to evolve. Phylogenetic analyses based on the sequence data from grass species support several relationships previously suggested by morphological work, but they are ambiguous about broad relationships within the family. IS - 1 JF - Mol. Biol. Evol. EP - 8 TI - Slipped-strand mispairing in a plastid gene: rpo{C}2 in grasses ({P}oaceae). VL - 11 SP - 1 KW - 5s KW - 8121278 KW - acid KW - aerobiosis KW - alcohol KW - alignment KW - amino KW - anaerobiosis KW - angiosperms KW - animals KW - arabidopsis KW - base KW - bibtex-import KW - c KW - cell KW - cereals KW - cerevisiae KW - chloroplasts KW - comparative KW - complex KW - conformation KW - cytochrome KW - data KW - dehydrogenase KW - diploidy KW - dna KW - dna-directed KW - electron KW - enzyme KW - evolution KW - expression KW - family KW - fungal KW - fungi KW - gene KW - genes KW - genetic KW - genetics KW - govt KW - group KW - haplotypes KW - homeobox KW - homeodomain KW - homology KW - humans KW - immunoglobulin KW - induction KW - iv KW - kinetics KW - mays KW - models KW - molecular KW - multigene KW - mutation KW - non-phs KW - non-us KW - nucleic KW - nucleus KW - oxygen KW - phs KW - phylogeny KW - plant KW - plants KW - plastids KW - poaceae KW - polymerases KW - polymorphism KW - primers KW - protein KW - proteins KW - region KW - regulation KW - repetitive KW - research KW - ribosomal KW - rna KW - saccharomyces KW - secondary KW - selection KW - sequence KW - sequences KW - species KW - specificity KW - structural KW - structure KW - study KW - support KW - transcription KW - transport KW - triticum KW - us KW - variable KW - variation KW - zea AU - Cummings, MP AU - King, LM AU - Kellogg, EA PY - 1994/Jan// ER -