The new paradigm of flow cell sequencing Export

@article{citeulike:2857577, abstract = {10.1101/gr.073262.107 DNA sequencing is in a period of rapid change, in which capillary sequencing is no longer the technology of choice for most ultra-high-throughput applications. A new generation of instruments that utilize primed synthesis in flow cells to obtain, simultaneously, the sequence of millions of different DNA templates has changed the field. We compare and contrast these new sequencing platforms in terms of stage of development, instrument configuration, template format, sequencing chemistry, throughput capability, operating cost, data handling issues, and error models. While these platforms outperform capillary instruments in terms of bases per day and cost per base, the short length of sequence reads obtained from most instruments and the limited number of samples that can be run simultaneously imposes some practical constraints on sequencing applications. However, recently developed methods for paired-end sequencing and for array-based direct selection of desired templates from complex mixtures extend the utility of these platforms for genome analysis. Given the ever increasing demand for DNA sequence information, we can expect continuous improvement of this new generation of instruments and their eventual replacement by even more powerful technology.}, author = {Holt, Robert A. and Jones, Steven J. M.}, citeulike-article-id = {2857577}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gr.073262.107}, citeulike-linkout-1 = {http://genome.cshlp.org/content/18/6/839.abstract}, citeulike-linkout-2 = {http://genome.cshlp.org/content/18/6/839.full.pdf}, citeulike-linkout-3 = {http://www.genome.org/cgi/content/abstract/18/6/839}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/18519653}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=18519653}, day = {1}, doi = {10.1101/gr.073262.107}, issn = {1088-9051}, journal = {Genome Research}, keywords = {454, genome, illumina, nextgensequencing, sequencing, solexa}, month = {June}, number = {6}, pages = {839--846}, posted-at = {2008-06-02 20:17:41}, priority = {2}, title = {The new paradigm of flow cell sequencing}, url = {http://dx.doi.org/10.1101/gr.073262.107}, volume = {18}, year = {2008} }

TY - JOUR ID - citeulike:2857577 L3 - citeulike-article-id:2857577 N2 - 10.1101/gr.073262.107 DNA sequencing is in a period of rapid change, in which capillary sequencing is no longer the technology of choice for most ultra-high-throughput applications. A new generation of instruments that utilize primed synthesis in flow cells to obtain, simultaneously, the sequence of millions of different DNA templates has changed the field. We compare and contrast these new sequencing platforms in terms of stage of development, instrument configuration, template format, sequencing chemistry, throughput capability, operating cost, data handling issues, and error models. While these platforms outperform capillary instruments in terms of bases per day and cost per base, the short length of sequence reads obtained from most instruments and the limited number of samples that can be run simultaneously imposes some practical constraints on sequencing applications. However, recently developed methods for paired-end sequencing and for array-based direct selection of desired templates from complex mixtures extend the utility of these platforms for genome analysis. Given the ever increasing demand for DNA sequence information, we can expect continuous improvement of this new generation of instruments and their eventual replacement by even more powerful technology. IS - 6 JF - Genome Research SN - 1088-9051 EP - 846 TI - The new paradigm of flow cell sequencing VL - 18 SP - 839 KW - 454 KW - genome KW - illumina KW - nextgensequencing KW - sequencing KW - solexa AU - Holt, Robert AU - Jones, Steven PY - 2008/06/01/ UR - http://dx.doi.org/10.1101/gr.073262.107 ER -