The cell wall galactomannan antigen from Malassezia furfur and Malassezia pachydermatis contains alpha-1,6-linked linear galactofuranosyl residues. Export

@article{citeulike:4408273, abstract = {Lipophilic yeasts of the genus Malassezia are associated with several skin diseases, such as pityriasis versicolor, Malassezia folliculitis, seborrheic dermatitis, and atopic dermatitis and are also increasingly associated with catheter-related fungemia. The cell wall components of pathogenic microorganisms behave as an antigen and/or ligand of the innate immune response. Live cells of Malassezia furfur and Malassezia pachydermatis did not react with the anti-alpha-1,2-mannoside antibody. However, they showed a strong hydrophobicity and reactivity with the anti-beta-1,3-glucan antibody compared to those of C. albicans. The cell wall polysaccharides of M. furfur and M. pachydermatis were isolated and their structures analyzed by (1)H and (13)C NMR experiments. Both polysaccharides were beta-1,6-linked linear galactofuranosyl polymers with a small amount of mannan. The presence of galactomannan on the Malassezia cells has previously not been described. The galactomannan did not react with the anti-Aspergillus fumigatus monoclonal antibody which has a specificity to the beta-1,5-linked galactofuranosyl residues. The anti-M. furfur antibody strongly reacted with the galactomannans of M. furfur and M. pachydermatis, but did not react with the galactomannans of Trichophyton rubrum, A. fumigatus, and Fonsecaea pedrosoi. The characteristics of the anti-M. furfur antibody suggest that there is a potential for the diagnostic application to Malassezia infections by antigen detection.}, author = {Shibata, Nobuyuki and Saitoh, Tomomi and Tadokoro, Yukari and Okawa, Yoshio}, citeulike-article-id = {4408273}, citeulike-linkout-0 = {http://dx.doi.org/10.1099/mic.0.029967-0}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19389777}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19389777}, day = {23}, doi = {10.1099/mic.0.029967-0}, issn = {1350-0872}, journal = {Microbiology (Reading, England)}, keywords = {basidiomycota, cell\_wall, smut}, month = {April}, posted-at = {2009-04-26 21:38:02}, priority = {2}, title = {The cell wall galactomannan antigen from Malassezia furfur and Malassezia pachydermatis contains {alpha}-1,6-linked linear galactofuranosyl residues.}, url = {http://dx.doi.org/10.1099/mic.0.029967-0}, year = {2009} }

TY - JOUR ID - citeulike:4408273 L3 - citeulike-article-id:4408273 N2 - Lipophilic yeasts of the genus Malassezia are associated with several skin diseases, such as pityriasis versicolor, Malassezia folliculitis, seborrheic dermatitis, and atopic dermatitis and are also increasingly associated with catheter-related fungemia. The cell wall components of pathogenic microorganisms behave as an antigen and/or ligand of the innate immune response. Live cells of Malassezia furfur and Malassezia pachydermatis did not react with the anti-alpha-1,2-mannoside antibody. However, they showed a strong hydrophobicity and reactivity with the anti-beta-1,3-glucan antibody compared to those of C. albicans. The cell wall polysaccharides of M. furfur and M. pachydermatis were isolated and their structures analyzed by (1)H and (13)C NMR experiments. Both polysaccharides were beta-1,6-linked linear galactofuranosyl polymers with a small amount of mannan. The presence of galactomannan on the Malassezia cells has previously not been described. The galactomannan did not react with the anti-Aspergillus fumigatus monoclonal antibody which has a specificity to the beta-1,5-linked galactofuranosyl residues. The anti-M. furfur antibody strongly reacted with the galactomannans of M. furfur and M. pachydermatis, but did not react with the galactomannans of Trichophyton rubrum, A. fumigatus, and Fonsecaea pedrosoi. The characteristics of the anti-M. furfur antibody suggest that there is a potential for the diagnostic application to Malassezia infections by antigen detection. JF - Microbiology (Reading, England) SN - 1350-0872 TI - The cell wall galactomannan antigen from Malassezia furfur and Malassezia pachydermatis contains {alpha}-1,6-linked linear galactofuranosyl residues. KW - basidiomycota KW - cell_wall KW - smut AU - Shibata, Nobuyuki AU - Saitoh, Tomomi AU - Tadokoro, Yukari AU - Okawa, Yoshio PY - 2009/04/23/ UR - http://dx.doi.org/10.1099/mic.0.029967-0 ER -