Assembly of the fungal SC3 hydrophobin into functional amyloid fibrils depends on its concentration and is promoted by cell wall polysaccharides. Export

@article{citeulike:5406363, abstract = {Class I hydrophobins function in fungal growth and development by self-assembling at hydrophobic-hydrophilic interfaces into amyloid-like fibrils. SC3 of the mushroom forming fungus Schizophyllum commune is the best studied class I hydrophobin. This protein spontaneously adopts the amyloid state at the water-air interface. In contrast, SC3 is arrested in an intermediate conformation at the interface between water and a hydrophobic solid such as PTFE (Teflon). This finding prompted us to study conditions that promote assembly of SC3 into amyloid-fibrils. Here, it is shown that SC3 adopts the amyloid state at the water-PTFE interface at high concentration (300 mug ml(-1)) and prolonged incubation (16 h). Moreover, it is shown that amyloid formation at both the water-air and water-PTFE interface is promoted by the cell wall components schizophyllan (beta-(1-3)beta-(1-6)-glucan) and beta-(1-3)-glucan. Hydrophobin concentration and cell wall polysaccharides thus contribute to the role of SC3 in formation of aerial hyphae and in hyphal attachment.}, author = {Scholtmeijer, Karin and de Vocht, Marcel L. and Rink, Rick and Robillard, George T. and Wosten, Han A.}, citeulike-article-id = {5406363}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M109.005553}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19654326}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19654326}, day = {4}, doi = {10.1074/jbc.M109.005553}, issn = {0021-9258}, journal = {The Journal of biological chemistry}, keywords = {basidiomycota, cell\_wall, fungi, hydrophobin, schizophyllum}, month = {August}, posted-at = {2009-08-10 22:26:06}, priority = {2}, title = {Assembly of the fungal SC3 hydrophobin into functional amyloid fibrils depends on its concentration and is promoted by cell wall polysaccharides.}, url = {http://dx.doi.org/10.1074/jbc.M109.005553}, year = {2009} }

TY - JOUR ID - citeulike:5406363 L3 - citeulike-article-id:5406363 N2 - Class I hydrophobins function in fungal growth and development by self-assembling at hydrophobic-hydrophilic interfaces into amyloid-like fibrils. SC3 of the mushroom forming fungus Schizophyllum commune is the best studied class I hydrophobin. This protein spontaneously adopts the amyloid state at the water-air interface. In contrast, SC3 is arrested in an intermediate conformation at the interface between water and a hydrophobic solid such as PTFE (Teflon). This finding prompted us to study conditions that promote assembly of SC3 into amyloid-fibrils. Here, it is shown that SC3 adopts the amyloid state at the water-PTFE interface at high concentration (300 mug ml(-1)) and prolonged incubation (16 h). Moreover, it is shown that amyloid formation at both the water-air and water-PTFE interface is promoted by the cell wall components schizophyllan (beta-(1-3)beta-(1-6)-glucan) and beta-(1-3)-glucan. Hydrophobin concentration and cell wall polysaccharides thus contribute to the role of SC3 in formation of aerial hyphae and in hyphal attachment. JF - The Journal of biological chemistry SN - 0021-9258 TI - Assembly of the fungal SC3 hydrophobin into functional amyloid fibrils depends on its concentration and is promoted by cell wall polysaccharides. KW - basidiomycota KW - cell_wall KW - fungi KW - hydrophobin KW - schizophyllum AU - Scholtmeijer, Karin AU - de Vocht, Marcel AU - Rink, Rick AU - Robillard, George AU - Wosten, Han PY - 2009/08/04/ UR - http://dx.doi.org/10.1074/jbc.M109.005553 ER -