Reprogramming of Xist against the pluripotent state in fusion hybrids. Export

@article{citeulike:5985054, abstract = {The fusion of somatic cells with pluripotent cells results in the generation of pluripotent hybrid cells. Because the 'memory' of somatic cells seems to be erased during fusion-induced reprogramming, genetic reprogramming is thought to be a largely unidirectional process. Here we show that fusion-induced reprogramming, which brings about the formation of pluripotent hybrids, does not always follow a unidirectional route. Xist is a unique gene in that it is reprogrammed to the state of somatic cells in fusion-induced pluripotent hybrids. In hybrids formed from the cell fusion of embryonal carcinoma cells (ECCs) with male neural stem cells (mNSCs), the Xist gene was found to be reprogrammed to the somatic cell state, whereas the pluripotency-related and tissue-specific marker genes were reprogrammed to the pluripotent cell state. Specifically, Xist is not expressed in hybrids, because the 'memory' of the somatic cell has been retained (i.e. mNSCs do not exhibit Xist expression) and that of the pluripotent cell erased (i.e. inactivation of the partially active Xist gene of ECCs, complete methylation of the Xist region). The latter phenomenon is induced by male, but not by female, NSCs.}, author = {Do, Jeong Tae T. and Han, Dong Wook W. and Gentile, Luca and Sobek-Klocke, Ingeborg and Wutz, Anton and Sch\"{o}ler, Hans R.}, citeulike-article-id = {5985054}, citeulike-linkout-0 = {http://dx.doi.org/10.1242/jcs.056119}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19843582}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19843582}, day = {20}, doi = {10.1242/jcs.056119}, issn = {1477-9137}, journal = {Journal of cell science}, keywords = {es, inactivation, stemness, x-chromosome, x-chromosome-inactivation, xci, xist}, month = {October}, posted-at = {2009-10-22 16:57:44}, priority = {5}, title = {Reprogramming of Xist against the pluripotent state in fusion hybrids.}, url = {http://dx.doi.org/10.1242/jcs.056119}, year = {2009} }

TY - JOUR ID - citeulike:5985054 L3 - citeulike-article-id:5985054 N2 - The fusion of somatic cells with pluripotent cells results in the generation of pluripotent hybrid cells. Because the 'memory' of somatic cells seems to be erased during fusion-induced reprogramming, genetic reprogramming is thought to be a largely unidirectional process. Here we show that fusion-induced reprogramming, which brings about the formation of pluripotent hybrids, does not always follow a unidirectional route. Xist is a unique gene in that it is reprogrammed to the state of somatic cells in fusion-induced pluripotent hybrids. In hybrids formed from the cell fusion of embryonal carcinoma cells (ECCs) with male neural stem cells (mNSCs), the Xist gene was found to be reprogrammed to the somatic cell state, whereas the pluripotency-related and tissue-specific marker genes were reprogrammed to the pluripotent cell state. Specifically, Xist is not expressed in hybrids, because the 'memory' of the somatic cell has been retained (i.e. mNSCs do not exhibit Xist expression) and that of the pluripotent cell erased (i.e. inactivation of the partially active Xist gene of ECCs, complete methylation of the Xist region). The latter phenomenon is induced by male, but not by female, NSCs. JF - Journal of cell science SN - 1477-9137 TI - Reprogramming of Xist against the pluripotent state in fusion hybrids. KW - es KW - inactivation KW - stemness KW - x-chromosome KW - x-chromosome-inactivation KW - xci KW - xist AU - Do, Jeong Tae AU - Han, Dong Wook AU - Gentile, Luca AU - Sobek-Klocke, Ingeborg AU - Wutz, Anton AU - Schöler, Hans PY - 2009/10/20/ UR - http://dx.doi.org/10.1242/jcs.056119 ER -